LyoVec Transfection Reagent
DNA Transfection Reagent
LyoVec was the first lyophilized cationic lipid-based
transfection reagent. It contains a novel proprietary cationic
lipid and is a combination of phosphonolipid DTCPTA & neutral
lipid DiPPE.
DTCPTA
DiPPE
Benefits
The benefits of using LyoVec™ include:
• Excellent efficiency
• Excellent cell line coverage including primary cells and
non-adherent cells
• Easy & Fast Procedure
• <15mins hands on time & Ready to use
• Stable complexes
• Low cytotoxicity-High cell viability
• Efficient in presence or absence of serum &
antibiotics
Comparison of transfection efficiency in various cells lines
after 48 hours with LyoVec v Reagent X. Reagent X is one of the
most utilized transfection reagents on the market & was
utilised according to the manufacturer's protocol.
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Applications
Applications of LyoVec™ include but are not restricted to:
• Routine DNA transfection - Over 90% transfection efficiency in
many cell lines
• Hard To Transfect Cells- Including primary & non-adherent
cells
• Stable & Transient transfections- Enables stable &
transient transfection
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Protocol
Please click here for further details and the standard protocol
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How To Order
LyoVec™ Ordering Information
|
Cat No
|
Product
|
Volume
|
Transfection No
|
|
lyec-12
|
LyoVec™
|
1 X 8ml
|
160 reactions
|
|
|
|
|
lyec-22
|
LyoVec™
|
1 X 18ml
|
360 reactions
|
|
|
|
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Publications
LyoVec™ has been referenced in many publications including:
Cell-type: Primary Leukocyte
Goodchild A. et al, 2009. Primary Leukocyte Screens for Innate
Immune Agonists. Journal of Biomolecular Screening, 14, pp.723 -
730.
Cell-type: HEK/Elam-luc
Sepulcre M.P. et al, 2009. Evolution of Lipopolysaccharide (LPS)
Recognition and Signaling: Fish TLR4 Does Not Recognize LPS and
Negatively Regulates NF-B Activation. The Journal of Immunology,
182, pp.1836 - 1845.
Cell-Type: U373MG
Terrasson J. et al, 2005. p73-Dependent Apoptosis through Death
Receptor: Impairment by Human Cytomegalovirus Infection. Cancer
Research, 65, pp.2787 - 2794.