C. elegans ORFeome version 1.1
Please note that the clone(s) being
sent have tested negative in our T1 phage contamination assay.
However these clones should still be handled with care as no phage
assay can be guaranteed to be 100% reliable.
THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED
AND ALSO THE ALIAS (PLATE AND WELL NUMBER ). THIS IS WHAT IS SHOWN
ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE
WHAT TO DO WITH YOUR CLONES WHEN YOU RECEIVE THEM
Plates- contain LB broth with 8% glycerol and kanamycin, are
sent on Dry Ice. These should be stored at -70°C
Individual clones pools have been streaked onto LB agar containing
kanamycin (100µg/ml). Please store them at 4°C (not in a freezer).
As soon as possible, transfer the culture to LB broth containing
kanamycin + 8% glycerol and incubate overnight at 37°C (do not try
to transfer single colonies, instead scoop out the whole bacterial
culture using a disposable inoculation loop). Incubate overnight at
37°C for subsequent freezing at -70°C. These frozen stocks can then
be used as templates for PCR amplification of ORFs, either directly
in colony PCRs or as plasmid DNA.
These clones are for research purposes
Please click on
to view vector map.
WormBase - http://www.wormbase.org/
Worfdb - http://worfdb.dfci.harvard.edu/
Acknowledgements and Subsequent Publication:
We are grateful to Dr. Marc Vidal (Dana-Farber Cancer Institute,
Harvard Medical School, Boston) for the gift of this library and
associated database and Dr Troy Moore (Open Biosystems) for
preparing a copy of the library.
Please acknowledge the Dana-Farber Cancer Institute and Source
BioScience LifeSciences in any resulting publications, clones
should be described in the paper in their ID form eg T22D1.3
Please also cite the following paper:
C. elegans ORFeome version 1.1: experimental verification of
the genome annotation and resource for proteome-scale protein
expression: Reboul J, Vaglio P, Rual JF, Lamesch P, Martinez M,
Armstrong CM, Li S, Jacotot L, Bertin N, Janky R, Moore T, Hudson
JR Jr, Hartley JL, Brasch MA, Vandenhaute J, Boulton S, Endress GA,
Jenna S, Chevet E, Papasotiropoulos V, Tolias PP, Ptacek J, Snyder
M, Huang R, Chance MR, Lee H, Doucette-Stamm L, Hill DE, Vidal
M.Nat Genet. 2003 May;34(1):35-41.