Drosophila Gene Collection (Berkeley)
DGCr3.0 NOW AVAILABLE!
The Berkeley Drosophila Genome Project (BDGP), a
consortium of the Drosophila Genome Center, has produced
over 240,000 ESTs derived from various tissues. The goal of the
consortium includes full-insert sequencing and assembly of
non-redundant cDNA clones. Annotated sequences of all Drosophila
transcripts, including products of alternative splicing, will
facilitate creation of a set of Drosophila Gateway clones
for use in proteomics and other functional genomics projects.
A set of ~11,000 clones has been assembled from ~90,000 EST
sequences, grouped or clotted, based on sequence identity from
their 5' ends. One representative from each clot was rearrayed and
its 5' and 3' ends sequenced, first to verify its identity, and
second to recluster on the basis of the 3' sequence.
Generation of the Release 3 annotation of the genome made
extensive use of our full-insert sequence data. In the course of
that effort, human curators identified a total of 1,860 clones that
have become the DGCr3. The DGCr3 currently includes clones chosen
to replace clones with truncated ORFs, clones for genes that are
not currently represented in the DGC, plus clones that represent
putative alternative splicing forms.
For more information please see BDGP webpage (http://www.fruitfly.org/DGC/index.html). We
also have 3 Databases for further information,
DGC 1,
DGC 2 and
DGC 3.
This resource is available as bacterial glycerol stocks and can
be ordered as individual clones, individual 384-well plates or
complete set of clones in 39 plates (DGCr1.0 = 17 plates and
DGCr2.0 = 16 plates & DGCr3.0 = 6).