Drosophila cDNA Library (NIH)
This library was constructed by Brian Oliver at the National
Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
which is part of the National Institutes of Health (NIH) at
Bethesda, MD, USA. Poly (A) + RNA was prepared from dissected
testes from 1-5 day old y w [67cl] Drosophila melanogaster flies,
after removal of the paragonia and inclusion of the ejaculatory
ducts and seminal vesicles. The size fractionated cDNA (1-6kb) was
directionally cloned in the Stratagene Uni-Zap XR vector using
EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision.
The host strain is E.coli SOLR.
The Resource Centre has approximately 3930 clones available from
plates (labelled bs) numbered 3- 24, 26- 37, 40- 42, 44- 46 and 49.
All plates except bs49 are available on one double-spotted high
density filter for screening by hybridisation.