OmicsLink™ ORF Expression Clones
Ready to Express
Source BioScience LifeSciences offers
over 20,000 human and 15,000 mouse full-length protein
coding ORF cDNA clones in multiple sets of vector systems with
various features ready for expression. These clones are suitable
for a variety of functional assays, protein expression and easy
purification in many cell types as well as in cell free expression
systems. Therefore, they constitute an excellent resource for large
scale functional genomics and proteomics studies.
Human Genes - Full Length Protein Coding ORF
OmicsLink™ ORF expression clones are
established following a stringent selection process by generation
of the entire coding open reading frame (ORF) of the most common
transcriptional variant of a gene and insertion into a modified
Gateway® Entry Vector using high fidelity cloning technologies.
Transfer of the ORF into target
expression vectors is possible by Invitrogen Corporation's Gateway®
Shuttling technology, as well as by classical restriction-ligation
cloning (Multiple Cloning Site present in the linker between
attachment site and start codon). Furthermore, and in contrast to
the classical Gateway® Technology, use of the patented RecJoin™
cloning strategy allows elimination of the extra 8 amino acids
encoded by attB site between 5' tag and the start codon ATG, if 5'
tag-ORF fusion expression clones are created. The same principle
applies to 3' fusion protein production.
OmicsLink™ Expression Clones allow
expression and functional analysis of target proteins in many hosts
such as E.coli, yeast, baculovirus and mammalian (including CHO)
cell lines as well as cell free transcription and translation
Fig. 1: 36 vector
types are available and ready for expression in bacterial,
mammalian, yeast and insect cells