UK Chicken Consortium EST Clones
This project to produce a large collection of EST clones
from the chicken was funded by the BBSRC and was directed by
colleagues at the Universities of Nottingham, Dundee and UMIST.
Sequencing was by Incyte Genomics Inc. All tissues were taken from
the White Leghorn (Layer) breed (Gallus domesticus). The Adult
Cerebrum, Adult Cerebellum and Adult Brain Other Parts tissues were
from the White Leghorn IAH strain of RPL Line 15I.
All embryonic tissues were from the White Leghorn 'Hisex' breed.
The remaining adult tissues (except muscle, chondrocyte, ovary,
pancreas and adipose) originate from White Leghorn Line 15
Note: IAH = Institute of Animal Health, RPL = Regional Poultry
Laboratory, East Lansing, Michigan. For more information please see
the Chicken EST Repository Homepage (http://www.chick.umist.ac.uk/).
21 tissues have been sequenced, with each tissue having multiple
cDNA libraries produced from different normalisation stringencies.
The majority of the clones are in pBluescript II KS+ (Stratagene)
in DH10B or DH10B-T1 resistant bacteria. EST sequences have been
produced from the 5' end of each clone.
Clones are currently supplied individually, with no limits on
the numbers that may be ordered. Clones can also be supplied
in our sequence
verifed and purified plasmid
formats. For supply of clones in other formats (e.g. whole
plates) please contact
To search for Chicken EST clones on our search and order
facility GenomeCube please use their ChEST ID number
(e.g. ChEST174m17 or 603157571F1).
To find out how to handle and store your clones