Expression Clones


Source BioScience LifeSciences' ready-to-use expression clones can be used directly for functional studies, assays and protein analysis. The expression clones contain the complete coding sequence or parts of the coding sequence for expression in several organisms. Different types of expression vectors can be selected carrying various tags with or without stop codon.

 

Clone Type

Species

Sequence Status

Contains Complete Coding Sequence

Amino Acid Changes Documented

Expression Verified

Comment

Reliability of Sequence Data

ORF Expression Clones

Human, Mouse, Yeast

fully sequenced

Yes

Yes

Some

flexible Gateway® System

Good

OmicsLink ORF Expression Clones (GeneCopoeia™)

Human

sequence-verified

Yes

No

Yes (in E.coli)

Fair

Partial ORF Expression Clones

Human

sequence-verified

No

No

Some

Poor

 

 

Sequence Status

Fully sequenced - full sequence is available

Sequence verified - partially sequenced from 3' or 5' or both ends

 

Contains Complete Coding Sequence

Yes -verification of the complete coding sequence based on the sequence data of the complete insert

No - insert contains only a part of the coding sequence

 

Expression Verified

All expression clones in pReceiver-B01 have been tested for expression in E.coli. Other expression clones with different features and for different host cell systems have not been tested for protein expression

 

Reliability of Sequence Data

Good - up to 0.0001% failure rate in sequence data

Fair - up to 2% failure rate in sequence data

Poor - up to 20% failure rate in sequence data

 

 

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