Mouse Genome Encyclopedia DNABook™
Over 60,000 full-length mouse cDNA clones (FANTOM2) are
available from the DNABook™. These are derived from 246 separate
cDNA libraries. FANTOM2 clones have a mean fragment size of 1.9Kbp
with a maximum size of 12.3Kbp. The clone set covers 90% of known
worldwide clones and represents the most extensive of condensed
mouse sets available.
RIKEN full-length cDNA clones
Full-length cDNA was collected using the Cap-trapper method and
a series of full-length cDNA technologies. Sequences were
determined using the high-throughput RISA sequencing pipeline.
Quality checks were implemented by re-sequencing all of the clones.
Derived sequence data for FANTOM2 clones are available in both DDBJ
and Genbank databases.
All FANTOM2 clones are provided in the DNABook™.
This resource forms an easily transportable bank of DNA, printed
on paper and bound as a book. DNA can be readily recovered from the
paper using PCR and/or transformation. See Kawai J and Hayashizaki
Y. (2003) "DNA Book" Genome Res. 13:1488-95.
Search DNABook™ for your gene
History of The Mouse Genome Encyclopaedia Project
RIKEN initiated the mouse genome encyclopaedia project in 1995,
at a time when genomic science was gaining prominence in the world
of research. Focusing on mouse full-length cDNA, RIKEN had two main
aims:
- To develop and establish a series of technologies and
analytical systems to be known as full-length cDNA technologies and
the RISA (RIKEN Integrated Sequence Analysis) system to create an
overview of the mouse transcriptome.
- The second aim was to establish the Mouse Genome Encyclopaedia
using the RISA system. The following bio-resources were instigated
for all of the mouse transcripts. Clone /sequence banks, plus a
functional annotation database for all mouse full-length
cDNAs.
- See Carninci P et al (2003) "Targeting a complex transcriptome: the
construction of the mouse full-length cDNA encyclopedia" Genome
Res. 13:1273-89.