Genomic Chicken BAC library
This chicken BAC library provided by Source BioScience
LifeSciences was constructed by Richard Crooijmans from Martien
Groenen's group at the Wageningen Agricultural University,
Department of Animal Sciences, Animal Breeding and Genetics Group,
The Netherlands in collaboration with the Texas A&M BAC Centre
of the Texas A&M University, College Station, Texas, USA . Work
on this resource is co-ordinated by Richard Crooijmans (richard.crooijmans@alg.vf.wau.nl)
and by ordering this library you are agreeing to your name being
passed on to him.
Female White Leghorn Chicken blood genomic DNA was cloned into
the pECBAC1 vector. This vector is a modification of pBeloBAC11 (
map, sequence)
containing a unique EcoR1 site, the EcoR1 site within the vector
having been removed leaving only one EcoR1 site within the lacZ
gene (Frijters et al Theor. Appl. Genet. [1997]
94:390-399). The average insert size is 130 kb. The library
consists of approx. 50,000 clones providing a 5.4-fold genomic
coverage.
The library is available from Source BioScience LifeSciences as
high density gridded filters for screening by hybridisation. The
set consists of 3 double spotted 22.2 x 22.2 cm filters.
( Crooijmans, RP et al Mamm.Genome [2000]
11:360-363).