Minimal Genomic Clone Set
Human Genomic Clone Set - Version 1.0
Description
Genomic Clones are a valuable resource for the functional
analysis of genes.
They can be used for a multitude of applications:
- FISH - Fluorescent In Situ Hybridization -
analysis of chromosomal rearrangements
- Matrix-CGH Comparative Genomic Hybridization - high
resolution analysis of genomic DNA
- Protein - DNA binding studies - promotor, enhancer,
silencer studies
- Subcloning of control elements
How to Find Genomic Clones of Interest
You can search for Genomic Clones by:
- Keywords or general IDs (like accession number, clone ID,...)
using GenomeCube™. This search type is currently limited to Homo
sapiens
- Downloading the complete annotated clone set information as
well as chromosomal subsets, listed below
Human
Minimal Genomic Clone Set: Chromosome 1 - 3
Human
Minimal Genomic Clone Set: Chromosome 4 - 6
Human
Minimal Genomic Clone Set: Chromosome 7 - 9
Human
Minimal Genomic Clone Set: Chromosome 10 - 12
Human
Minimal Genomic Clone Set: Chromosome 13 - 15
Human
Minimal Genomic Clone Set: Chromosome 16 - 18
Human
Minimal Genomic Clone Set: Chromosome 19 - 21
Human
Minimal Genomic Clone Set: Chromosome 22 - XY
Annotated
Clone List
Clone Selection Criteria
The Clone Set was developed on basis of NCBI's genome assembly and the Ensembl
annotation. The current clone set consists of 25,130 clones.
The majority (22,898 clones, 91%) of which have been completely
sequenced during the human genome sequencing project. Gaps
were covered by 2,232 end-sequenced clones as far as such clones
were available. We focused on publicly available BAC and PAC clones
and completed the set where necessary with cosmids. The overall
average insert size is approx 130 kb.
Product Range
- Clone Collection in microtiter plates
- Customized subsets
Quality Control
- Viabilty
- Contamination check (absence of contaminations of
bacteriophages and non-E. coli microorganims)
- Partly sequence-verified (end-sequencing of more than 7,400
clones)
References
Candidate Genes in Breast Cancer Revealed by Microarray-Based
Comparative Genomic Hybridization of Archived Tissue
Cancer Research 65, 439-447, January 15,
2005
Detection of Chromosomal Imbalances in Retinoblastoma by
Matrix-Based Comparative Genomic Hybridization
CANCER 43:294-301 (2005)