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I.M.A.G.E.

 

The I.M.A.G.E. (Integrated Molecular Analysis of Genomes and their Expression) Consortium's represents the world's largest public cDNA collection. Over nine million individual cDNA clones are available - free of restriction - for further studies.

 

Source BioScience is an official distributor of the I.M.A.G.E & Mammalian Gene Collection (MGC) clone collections. We distribute high quality, full-length sets and clones which can be sequence verified within our in-house facilities.

 

For individual I.M.A.G.E. clone orders please use our GenomeCube® search engine. Please don't forget to order our purified plasmids to speed your down-stream analyses!

 

 

Complete library sets or individual plates can be ordered using our Quick Buy Tool.

 

 

 

 

 

MGC, XGC, ZGC Predicted Full-Length (IRAK, IRAL, IRAM)

These human, mouse, rat, zebrafish, Xenopus laevis and X. tropicalis clones have been computationally predicted to be full-length based on homology with known genes, and several other methods of prediction for novel genes. In 384-well format, the IRAK rearray contains ampicillin-resistant clones and the IRAL rearray contains chloramphenicol-resistant clones, and IRAM contains kanamycin-resistant clones. Each plate contains clones from multiple libraries, but from one species and one vector only. These clones are being sequence-verified and fully sequenced by the MGC Consortium. To access the full-length sequences, search Genbank (not dbEST) using the following format: "MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.

 

Mammalian Gene Collection (MGC) Verified Full-Length Human (IRAT, IRAU)

This is a subset of the IRAK/IRAL MGC predicted full-length set; these clones have been sequence-verified to match the EST already in dbEST, and have been fully sequenced to high accuracy by the MGC Consortium (full-length sequences are submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate containing clones from one species and vector only. Rearray names are  IRAT (human clones, ampicillin-resistant vectors) and  IRAU (human clones, chloramphenicol-resistant vectors. Periodic updates will be made as more full-length sequences are submitted. To access the full-length sequences, search Genbank (not dbEST) using the following format: "MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.

 

Mammalian Gene Collection (MGC) Verified Full-Length Mouse (IRAV, IRAW)

This is a subset of the IRAK/IRAL MGC predicted full-length set; these clones have been sequence-verified to match the EST already in dbEST, and have been fully sequenced to high accuracy by the MGC Consortium (full-length sequences are submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate containing clones from one species and vector only. Rearray names are  IRAV (mouse clones, ampicillin-resistant vectors) and  IRAW (mouse clones, chloramphenicol-resistant vectors). Periodic updates will be made as more full-length sequences are submitted. To access the full-length sequences, search Genbank (not dbEST) using the following format: "MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.

 

Mammalian Gene Collection (MGC) Verified Full-Length Rat (IRBP, IRBQ)

This is a subset of the IRAK/IRAL MGC predicted full-length set; these clones have been sequence-verified to match the EST already in dbEST, and have been fully sequenced to high accuracy by the MGC Consortium (full-length sequences are submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate containing clones from one species and vector only. Rearray names are  IRBP (rat clones, ampicillin-resistant vectors) and  IRBQ (rat clones, chloramphenicol resistant vectors). Periodic updates will be made as more full-length sequences are submitted. To access the full-length sequences, search Genbank (not dbEST) using the followingformat: "MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.

 

XGC Verified Full-Length Xenopus (IRBG, IRBH, IRBN)

This is a subset of the IRAK/IRAL XGC predicted full-length set for X. laevis and X. tropicalis; these clones have been sequence-verified to match the EST already in dbEST, and have been fully sequenced to high accuracy by XGC (full-length sequences are submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate containing clones from one species and vector only. Rearray names are  IRBG (X. laevis, chloramphenicol-resistant vectors),  IRBH (X. laevis, ampicillin resistant-vectors) and  IRBN (X. tropicalis, ampicillin-resistant vectors). Periodic updates will be made as more full-length sequences are submitted. To access the full-length sequences, search Genbank (not dbEST) using the following format: "MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.

 

ZGC Verified Full-Length Zebrafish (IRBO)

This is a subset of the IRAK/IRAL ZGC predicted full-length set for zebrafish; these clones have been sequence-verified to match the EST already in dbEST, and have been fully sequenced to high accuracy by ZGC (full-length sequences are submitted to the primate division of Genbank, NOT dbEST). This rearray is in 96-well format, with each plate containing clones from one species and vector only. Rearray name is  IRBO (zebrafish clones, ampicillin-resistant vectors). Periodic updates will be made as more full-length sequences are submitted. To access the full-length sequences, search Genbank (not dbEST) using the following format: "MGC:MGC_id" to return individual sequences or MGC [kywd] for all sequences.

 

Human Genes - Lymphochip (IRAY, IRAZ, IRBA, IRBB)

This set of 2,500 known human genes (1,415 unique genes, 1,100 of them represented by two independent clones) has been rearrayed in 96-well format. Each plate contains one vector only.  IRAY is a non-redundant rearray of ampicillin-resistant clones,  IRAZ is a non-redundant rearray of chloramphenicol-resistant clones,  IRBA is a non-redundant rearray of ampicillin-resistant clones that completely overlaps the genes represented in IRAY, and  IRBB is a non-redundant rearray of chloramphenicol-resistant clones that completely overlaps the genes represented in IRAZ.

 

Human GPCR Genes (IRBF, IRBI)

The full open reading frames of individual GPCR genes have been PCR-amplified and cloned in 96-well format. Originally the rearray names were  IRBF (ampicillin-resistant, from the Guthrie cDNA Resource Centre) and  IRBI (ampicillin-resistant, from the GPCR Consortium, but more recently clones from Guthrie and GPCR Consortium have been combined into single plates under the IRBI collection. The origin of each clone can still be tracked with the Guthrie clones originating from library NIH_MGC_146 and the GPCR Consortium clones originating from library NIH_MGC_145. For specific information, please refer to the appropriate IMAGE ftp-site.

 

Human Genes - Glioma-Related (IRAX)

A set of 5,500 mostly unique human ampicillin-resistant clones representing glioma-related genes (rearray name  IRAX). Each plate contains one vector only except for plate 1 which contains a small number of clones from three different vectors. For specific information, please refer to the appropriate IMAGE ftp-site.

 

Mouse Genes - Lymphochip (IRAP)

A set of unique ampicillin-resistant clones, representing 13,000 mouse genes (rearray name  IRAP. Sequence-verification is planned. Each plate contains one vector only. For specific information, please refer to the appropriate IMAGE ftp-site.

 

Mouse Pancreas Genes (IRBD, IRBE)

This is a set of 7,000 mouse genes. Rearray  IRBD contains ampicillin-resistant clones, rearray  IRBE contains kanamycin-resistant clones. For specific information, please refer to the appropriate IMAGE ftp-site .

 

University of Iowa Rat UniGene Set (IRAD)

The IRAD rearray contains ampicillin-resistant clones in 96-well format. It was prepared in the Soares laboratory at the University of Iowa based on Unigene clustering.