Subsets of the I.M.A.G.E. Consortium resources
The I.M.A.G.E. resources are comprised of a large number of
arrays of cDNA libraries from RNA made on different species and
tissues. Six subsets of clones from these arrays have been picked
and are available.
The Mammalian Gene Collections (three human, three mouse, two
rat and one cow).
This is described here.
The Xenopus Gene Collections (two X. laevis, and one X.
tropicalis).
This is decribed here.
The Zebrafish Gene Collection (Four Libraries).
This is decribed here.
The Mouse Lymphochip subset
I.M.A.G.E. Consortium calls this subset IRAP. The clones were
selected on the basis of an association with lymphoid tissue. The
subset contains 12,480 different clones representing 12,480 genes.
The clones have not been fully sequenced.
The inserts are harboured in ampicillin-resistant vectors. There
are 130 (96-well) plates. On any one plate, just one vector has
been employed. This resource is distributed as a single set.
The identity of the clones is given here.
The Human Lymphochip subset
The I.M.A.G.E. Consortium calls this subset IRAY, IRBA, IRAZ and
IRBB. The clones were selected on the basis of an association with
lymphoid tissue. The subset contains 2,517 different clones. The
clones have not been fully sequenced.
The subset contains one representative of 313 genes. The subset
contains two representative clones for 1,102 genes.
The inserts are harboured in ampicillin-resistant vectors for
the plates in the IRAY and IRBA sets. The inserts are harboured in
chloramphenicol-resistant vectors for the plates in the IRAZ and
IRBB sets. There are 35 (96 well) plates. On any one plate, just
one vector has been employed. This resource is distributed as a
single set.
IRAY - 96-well plates numbered 1 -
17 (1280 clones)
IRBA - 96-well plates numbered 1 -
12 (967 clones)
IRAZ - 96-well plates numbered 1 -
3 (135 clones)
IRBB - 96-well plates numbered 1 -
3 (135 clones)
The identity of the clones is given here.
The Human glioma-related subset
The I.M.A.G.E. Consortium calls this subset IRAX. The clones were
selected on the basis of an association with gliomas. These are a
diverse group of brain tumors that arise from the normal glial
cells of the brain. The subset contains 5,664 clones usually
representing different genes. The clones have not been fully
sequenced.
The inserts are harboured in ampicillin-resistant vectors. There
are 59 (96 well) plates. On any one plate (except plate 1), just
one vector has been employed. Plate 1 contains three different
vectors. This resource is distributed as a single set.
The identity of the clones is given here.
Mouse pancreas genes:
The I.M.A.G.E. Consortium calls this subset IRBD and IRBE. This
set of 7000 mouse genes (IRBD (amp-resistant) and IRBE
(kanamycin-resistant) has been rearrayed by the K. Kaestner
laboratory (University of Pennsylvania) in 96-well format. Each
plate contains clones from one vector only. There are 4
partially-full plates.A flatfile containing all the data
(IRBD_IRBE.preSV.data) can be found on the anonymous ftp site at ftp://image.hudsonalpha.org/image/rearrayed_plates/IRBD_IRBE.preSV.data.This
resource is available as a single set. Individual clones can be
ordered on our standard IMAGE order form.