The Mammalian Gene Collection
The Mammalian
Gene Collection (MGC) project is an NIH initiative. The goal of
the project is to make available a complete set of these
full-length cDNA clones in human, rat, mouse and cow.
The MGC plan of work is:
-
To produce libraries enriched for full-length cDNA;
-
To sequence the 5' and 3' ends of 768 clones in each library for
library validation;
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To array and sequence from each validated library the 5' ends of
10,000 to 30,000 clones. Each of these clones is given an
I.M.A.G.E. ID and becomes part of the main I.M.A.G.E. resource
(LLAM and LLCM clones);
-
To apply several algorithms to analyze the 5' sequences to
identify clones containing putative N-terminal coding regions. The
clones identified are rearrayed into 384-well plates by the I.M.A.G.E.
Consortium at LLNL. These plates are labelled with the prefix
IRAK, IRAL or IRAM on the I.M.A.G.E. web site and each clone retains its
unique IMAGE ID;
-
Only those clones confirmed by sequencing to harbour full-length
ORFs are assigned a unique MGC identifier (e.g. MGC_15287 or its
synonym MGC:15287) in addition to their original IMAGE ID. These
sequences are deposited in GenBank.
-
To rearray clones with a unique MGC_ID into 96-well plates.
In summary a Mammalian Gene Collection (MGC) clone is identified
by:
Description of rearrayed plates containing sequenced
full-length MGC clones:
| IRAT |
Human |
Vectors confer
ampicillin resistance |
| IRAU |
Human |
Vectors confer
chloramphenicol resistance |
| IRCM |
Human |
Vectors confer
Kanamycin resistance |
| IRAV |
Mouse |
Vectors confer
ampicillin resistance |
| IRAW |
Mouse |
Vectors confer
chloramphenicol resistance |
| IRCL |
Mouse |
Vectors confer
Kananmycin resistance |
| IRBP |
Rat |
Vectors confer
ampicillin resistance |
| IRBQ |
Rat |
Vectors confer
chloramphenicol resistance |
| IRCJ |
Cow |
Vectors confer
amplicillin resistance |
NB: In each of these subsets all clones on one plate share the
same vector ( IRAT, IRAU, IRCM, IRAV, IRAW, IRCL, IRBP, IRBQ and IRCJ). Details of
the vectors used can be found here
During a recent sequencing effort, the IMAGE consortium has
produced QC statistics for some of the rearrayed libraries (click here)
Clones from the six subsets can be ordered as individual clones
(streaks on agar) and 96-well plates (frozen bacterial stocks in
glycerol).
Clones are ordered using the IMAGE ID on the standard I.M.A.G.E.
order form (you cannot use the MGC_ID). If the clone ordered forms
part of a MGC subset in the Source BioScience LifeSciences
collection, you will receive the clone streaked out from IRAT,
IRAU, IRCM, IRAV, IRAW, IRCL, IRBP, IRBQ and IRCJ plates. Such
clones will also have an MGC_ID which will be given on the email
confirmation of your order. If a clone has an MGC_ID but is not yet
part of the subset plates received by Source BioScience
LifeSciences the clone will be streaked out from the main
I.M.A.G.E. collection.
For a list of the current plates available in each MGC subset
please click here.
Publications
Strausberg RL, Feingold EA, Klausner RD & Collins FS(1999).
The Mammalian Gene Collection. Science 286: 455-457
MGC (Mammalian Gene Collection) Program Team (2002). Generation
and Initial Analysis of more than 15000 Full-Length Human and Mouse
cDNA sequences. PNAS 99(26): 16899-16903