The Xenopus Gene Collection
Gene Collection (XGC) project is trans-NIH initiative, which
was launched, as a subproject of the existing Mammalian Gene
Collection. The general approach is to acquire or construct
high-quality cDNA libraries. The libraries are screened by
sequencing the 5' end of the clones. Putative full open reading
frames clones are identified, and finally, selected clones are
full-length sequence verified. The goal of the project is to make
available a complete set of these full-length cDNA clones in
Xenopus laevis and Xenopus tropicalis.
The XGC plan of work is:
The generation of libraries from different stages of development
and adult tissues.
Sequencing of ~3,000 to 10,000 5' and 3' ESTs from each
All sequences are deposited into GenBank. The corresponding
clones are available through the IMAGE clone distribution network
and are fully accessible to the community.
Analysis of all available sequences into UniGene clusters.
Selection of candidate complete open reading frame (ORF) clones
and their sequencing.
In summary a Xenopus Gene Collection (XGC) clone is identified
Description of rearrayed plates containing sequenced
full-length MGC clones:
NB: In each of these subsets all clones on one plate share the
same vector ( IRBH, IRBG and IRBN).
Details of the vectors used can be found here
During a recent sequencing effort, the IMAGE consortium has
produced QC statistics for some of the rearrayed libraries (click here)
Clones from the three subsets can be ordered as individual
clones (streaks on agar) and 96-well plates (frozen bacterial
stocks in glycerol).
Clones are ordered using the IMAGE ID on the standard I.M.A.G.E.
order form (you cannot use the XGC_ID). If the clone ordered forms
part of a XGC subset in the Source BioScience LifeSciences
collection, you will receive the clone streaked out from IRBH,
IRBG, or IRBN plates. Such clones will also have an XGC_ID which
will be given on the email confirmation of your order. If a clone
has an XGC_ID but is not yet part of the subset plates received by
Source BioScience LifeSciences the clone will be streaked out from
the main I.M.A.G.E. collection.