The Zebrafish Gene Collection
Gene Collection (ZGC) project is an NIH initiative which was
launched in April 2002, as a subproject of the existing Mammalian
Gene Collection. The general approach is to acquire or construct
high-quality cDNA libraries. The libraries are screened by
sequencing the 5' end of the clones. Putative full open reading
frames are identified, and finally, selected clones are full-length
sequence verified. The goal of the project is to make available a
complete set of these full-length cDNA clones in Zebrafish.
The ZGC plan of work is a phased project:
Initially, the ZGC will collect 5' sequences from clones in
various libraries over a 3-month period.
At the end of that time, an external panel of scientists will
review the list of all the putative full-length clones eligible for
full-length sequencing and prioritize those for sequencing.
This process will then be repeated quarterly.
In addition, the ZGC will collaborate with other researchers who
have significant existing collections of putative full-length
clones to sequence those clones.
In summary a Zebrafish Gene Collection (ZGC) clone is identified
Description of rearrayed plates containing sequenced
full-length MGC clones:
NB: In each of these subsets all clones on one plate share the
same vector ( IRBO, IRBV, IRCX, IRCY). Details of
the vectors used can be found here
During a recent sequencing effort, the IMAGE consortium has
produced QC statistics for some of the rearrayed libraries (click here)
Clones from this subset can be ordered as individual clones
(streaks on agar) and 96-well plates (frozen bacterial stocks in
Clones are ordered using the IMAGE ID on the standard I.M.A.G.E.
order form (you cannot use the ZGC_ID). If the clone ordered forms
part of a ZGC subset in the Source BioScience LifeSciences
collection, you will receive the clone streaked out from IRBO,
IRCX, IRCY and IRBV plates. Such clones will also have a ZGC_ID
which will be given on the email confirmation of your order. If a
clone has a ZGC_ID but is not yet part of the subset plates
received by Source BioScience LifeSciences the clone will be
streaked out from the main I.M.A.G.E. collection.