What are standard, sequence verified and purified plasmid clones? | Source BioScience

Source BioScience offer 3 different formats when purchasing selected* clones, standard, sequence verified and purified plasmid. 

*Please note that not all of our clones are available in all 3 formats. The type of format available for each clone is indicated in the product information on GenomeCube.

Standard format clones

You will receive:

  • Bacterial clone (as stated in product information)
  • Data sheet

It should be noted that Source BioScience excludes any warranty pertaining to the accuracy of the sequence for clone orders where the client chooses to reject the offered sequence verification option, as the clones are supplied as-is, direct from the original library.

Sequence verified

You will receive:

  • Bacterial clone (as stated in product information)
  • Data sheet
  • BLAST report

Source BioScience recommends that the purchaser requests sequence-verification using the Source BioScience Clone Identity Verification Service at the time of ordering. This can be requested by selecting the sequence verified or purified plasmid clone format during the ordering process.

These clones are end sequenced before they are sent to you. If the requested clone does not match the expected sequence, Source BioScience will not apply any charge and will contact the purchaser to confirm if they wish to cancel the order.

This risk free option ensures that you have the right clone, removing the chance of receiving one of the wrong clones that are inherent in some libraries and can also save you time from having to sequence the clone yourself.

Purified plasmid

You will receive:

  • Bacterial clone (as stated in product information)
  • End sequenced verified plasmid mini-prep*
  • Data sheet
  • BLAST report

The purified plasmid is high quality DNA for use in PCR amplification, DNA sequencing, transformation and downstream enzyme reactions

* Typically 3-5 μg plasmid DNA, depending on copy number

 

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