Vectors in the FANTOM™ 3 Set


Different linkers were used to clone into the same vector during the library construction. Therefore, to select the correct vector, please refer to both to re-array prefix and the primers used to construct the cDNA.

Whilst re-arraying the clones for full-length sequencing, RIKEN grouped clones into sets that have the same vector and primers in common. Please refer to the following the table; clicking on the hyperlinks will display further details of the vector.

Vector information

Vector

Re-array PREFIX 1st-primer for cDNA construction 2nd-primer for cDNA construction g-tail poly-A
Modified Bluescript1 ZA 1st-NX 2nd-AT + +
Modified Bluescript1 ZX 1st-BS 2nd-NX/X + +
pFLCI PX
PY (strain="NOD")
PZ (host="DH5a")
1st-BS 2nd-NX/X + +
pFLCI PK
PT(1st-primer="1st-BG tag", strain="Transgenic hTNF")
PI(1st-primer="1st-BG tag", strain="CD-1")
PL(1st-primer="1st-BG tag")
1st-BS 2nd-linker - +
pFLCI PH(1st-primer="1st-BG tag", strain="Transgenic hTNF") 1st-BS 2nd-hairpin-linker - +
pFLCI PR
PN(strain="NOD")
1st-BS 2nd-linker - +
pFLCI PS
PM(strain="NOD")
1st-BS(Short Poly-A) 2nd-linker - short
pFLCII FX 1st-BS 2nd-NX/X + +
pFLCII FK 1st-BS 2nd-linker - +
pFLCII FL 1st-BS(Short Poly-A) 2nd-linker - short
pFLCIII FU
FV(1st-primer="1st-BG tag")
1st-BS 2nd-NX/X + +
pFLCIII/pBAC FS(1st-primer="1st-BG tag")
FW(1st-primer="1st-BG tag")
FY(1st-primer="1st-BG tag", host="JW366")
1st-BS 2nd-linker - +
pFLCIII FT(1st-primer="1st-BG tag") 1st-BS 2nd-hairpin-linker - +