Mouse Genome Encyclopedia DNABook™

Over 60,000 full-length mouse cDNA clones (FANTOM2) are available from the DNABook™. These are derived from 246 separate cDNA libraries. FANTOM2 clones have a mean fragment size of 1.9Kbp with a maximum size of 12.3Kbp. The clone set covers 90% of known worldwide clones and represents the most extensive of condensed mouse sets available.

 

RIKEN full-length cDNA clones

Full-length cDNA was collected using the Cap-trapper method and a series of full-length cDNA technologies. Sequences were determined using the high-throughput RISA sequencing pipeline. Quality checks were implemented by re-sequencing all of the clones. Derived sequence data for FANTOM2 clones are available in both DDBJ and Genbank databases.

All FANTOM2 clones are provided in the DNABook™.

This resource forms an easily transportable bank of DNA, printed on paper and bound as a book. DNA can be readily recovered from the paper using PCR and/or transformation. See Kawai J and Hayashizaki Y. (2003) "DNA Book" Genome Res. 13:1488-95.

 

Search DNABook™ for your gene

Mouse Genome Encyclopedia

 

History of The Mouse Genome Encyclopaedia Project

RIKEN initiated the mouse genome encyclopaedia project in 1995, at a time when genomic science was gaining prominence in the world of research. Focusing on mouse full-length cDNA, RIKEN had two main aims:

 

  1. To develop and establish a series of technologies and analytical systems to be known as full-length cDNA technologies and the RISA (RIKEN Integrated Sequence Analysis) system to create an overview of the mouse transcriptome.
  2. The second aim was to establish the Mouse Genome Encyclopaedia using the RISA system. The following bio-resources were instigated for all of the mouse transcripts. Clone /sequence banks, plus a functional annotation database for all mouse full-length cDNAs.
  3. See Carninci P et al (2003) "Targeting a complex transcriptome: the construction of the mouse full-length cDNA encyclopedia" Genome Res. 13:1273-89.