What to do with your clones when you receive them
Plates- contain LB broth with 8% glycerol and ampicillin (50 µg/ml) are sent on Dry Ice. These should be stored at -70°C.
Individual clones have been streaked onto LB agar containing ampicillin (50 µg/ml). Please store them at 4°C (not in a freezer).
As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.
A selection of at least 10 single colonies should be picked and grown in LB broth containing antibiotic + 8% glycerol for subsequent freezing at -70°C. These frozen stocks can then be re-grown for plasmid isolation and purification.
Streaking out for single colonies is essential in case of contamination or cross-contamination, or if modifications of the original clone have taken place as the library has been replicated. If there is a problem, the more single colonies you streak out, the more likely it is that you can retrieve the original construct. You can also isolate the original clone from a contaminated sample by following this procedure.
The GPCR clone collection has been produced by Guthrie cDNA Resource Center (http://www.guthrie.org/) which is a non-profit organization. The cDNA clones have been produced in pcDNA3.1+ and pDNR-1r vectors. Please click on the links below for vector information:
Please go to the following website for full listing of available clones - http://www.imageconsortium.org/ftp/rearrayed_plates/IRBF-IRBI.preSV.data
Please acknowledge Guthrie cDNA Resource Center (http://www.guthrie.org/) in any resulting publications.