Please note that the plates being sent have tested negative in our phage contamination assay. However these plates should still be handled with care as no phage assay can be guaranteed to be 100% accurate.
Plates have been replicated in LB broth containing 8% glycerol and 50µg/ml ampicillin. 59 plates (96-well format) have been shipped frozen in dry ice and should be placed in a -70°C freezer on arrival. To make further copies replicate in the above media and incubate overnight at 37°C.
These frozen stocks can be re-grown for plasmid isolation and purification or PCR amplification.
All clones on one plate share the same vector, except for plate 1 which contains 3 different vectors.
The following vectors have been used:
pBluescript SK- pCMV-SPORT6 pT7T3D-PacI lafmid BA pAMP1
Full details on these vectors can be obtained from here
Full details on each clone are available here