I.M.A.G.E. human glioma-related subset plates: IRAX

Please note that the plates being sent have tested negative in our phage contamination assay. However these plates should still be handled with care as no phage assay can be guaranteed to be 100% accurate.

What to do with your plates when you receive them


Plates have been replicated in LB broth containing 8% glycerol and 50µg/ml ampicillin.
59 plates (96-well format) have been shipped frozen in dry ice and should be placed in a -70°C freezer on arrival.
To make further copies replicate in the above media and incubate overnight at 37°C.

These frozen stocks can be re-grown for plasmid isolation and purification or PCR amplification.

 All clones on one plate share the same vector, except for plate 1 which contains 3 different vectors.

The following vectors have been used:

pBluescript SK-
lafmid BA

Full details on these vectors can be obtained from here

Full details on each clone are available here