I.M.A.G.E. human lymphochip subset plates: IRAY, IRBA, IRAZ AND IRBB

Please note that the plates being sent have tested negative in our phage contamination assay. However these plates should still be handled with care as no phage assay can be guaranteed to be 100% accurate..

What to do with your plates when you receive them

IRAY and IRBA

Plates have been replicated in LB broth containing 8% glycerol and 50µg/ml ampicillin.
17 IRAY plates and 12 IRBA plates (96-well format) have been shipped frozen in dry ice and should be placed in a -70°CC freezer on arrival.
To make further copies replicate in the above media and incubate overnight at 37°C.

 

IRAZ and IRBB

Plates have been replicated in LB broth containing 8% glycerol and 27µg/ml chloramphenicol.
3 IRAZ plates and 3 IRBB plates (96-well format) have been shipped frozen in dry ice and should be placed in a -70°C freezer on arrival.
To make further copies replicate in the above media and incubate overnight at 30°C.


These frozen stocks can be re-grown for plasmid isolation and purification or PCR amplification.

All clones on one plate share the same vector.

The following vectors have been used:


pBluescript SK
pBluescript SK-
pBluescript R
lafmid BA
pCMV-SPORT4
pCMV-SPORT6
pCMV-SPORT6.ccdb
pSPORT1
pT7T3D-PacI
pOTB7
pOTB7a
pDNR-LIB
pAMP1
pAMP10

 

Full details on these vectors can be obtained from here

Full details on each clone are available here