Please note that the plates being sent have tested negative in our phage contamination assay. However these plates should still be handled with care as no phage assay can be guaranteed to be 100% accurate.
Plates have been replicated in LB broth containing 8% glycerol and 50µg/ml ampicillin. 130 plates (96-well format) have been shipped frozen in dry ice and should be placed in a -70°C freezer on arrival. To make further copies replicate in the above media and incubate overnight at 37°C.
These frozen stocks can be re-grown for plasmid isolation and purification or PCR amplification.
All clones on one plate share the same vector. The following vectors have been used:
pBluescribe modified pBluescript SK+ pCMV-SPORT2 pCMV-SPORT6 pME18S-FL3 pSPORT1 pT7T3D-Pac pZL1
Full details on these vectors can be obtained here
Full details on each clone are available here