I.M.A.G.E. mouse pancreas subset IRBD and IRBE

Please note that the plates/clones being sent have tested negative in our phage contamination assay. However these should still be handled with care as no phage assay can be guaranteed to be 100% accurate.

 

THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER ). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE. If you wish to translate from our plate to the original IMAGE ID, please use our plate conversion tool. Entering the ID as eg BD1

What to do with your plates/clones when you receive them

IRBD

Clones have been grown on LB agar containing 50µg/ml ampicillin, these should be placed in a 4°C. fridge.As soon as possible, restreak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.

Plates have been replicated in LB broth containing 8% glycerol and 50µg/ml ampicillin.
71 plates (96-well format) have been shipped frozen in dry ice and should be placed in a -70°C freezer on arrival.To make further copies replicate in the above media and incubate overnight at 37°C.

Plates 1-4 are in pBluescript II SK, plates 5-71 are in pSport I.

IRBE

Clones have been grown on LB agar containing 50µg/ml kanamycin, these should be placed in a 4°C. fridge.As soon as possible, restreak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies

Plates have been replicated in LB broth containing 8% glycerol and 50µg/ml kanamycin. 6 plates (96-well format) have been shipped frozen in dry ice and should be placed in a -70°C freezer on arrival.

To make further copies replicate in the above media and incubate overnight at 37°C.

All 6 plates are in pZero2 vector.

These frozen stocks can be re-grown for plasmid isolation and purification or PCR amplification.

Clones - A selection of at least 10 single colonies should be picked and grown in LB broth containing the appropriate antibiotic + 8 % glycerol for subsequent freezing at -70°C.

These frozen stocks can then be regrown for plasmid isolation and purification.

Streaking out for single colonies is essential in case of contamination or cross-contamination, or if modifications of the original clone have taken place as the library has been replicated. If there is a problem, the more single colonies you streak out, the more likely it is that you can retrieve the original construct. You can also isolate the original clone from a contaminated sample by following this procedure.

All clones on one plate share the same vector. Full details on these vectors can be obtained from here

Full details on each clone are available here