RPCI-11 Human Male BAC clones

The RP11 Human Male BAC library was generated by the BACPAC Resource Center (BPRC) at the Children’s Hospital Oakland Research Institute by Kazutoyo Osoegawa. The work was funded by the National Human Genome Research Institute (NHGRI, NIH).

Male blood was partially digested using a combination of EcoRI and EcoRI Methylase. The size selected DNA was then cloned into the pBACe3.6 vector). For Segment 5 the same DNA was partially digested with MboI. This was then size selected and ligated into the cloning vector pTARBAC1. The products were then transformed into E.coli DH10B. The clones have been arrayed into 384 well plates.

Library Segment

Plate Numbers

Vector

Total Clone Number

1

1-288

pBACe3.6

108,499

2

289-576

pBACe3.6

109,496

3

577-864

pBACe3.6

109,657

4

865-1152

pBACe3.6

109,382

5

1153-1440

pTARBAC1

106,763

Total Library

 

 

543,797

Average insert length (link http://bacpac.chori.org/rpci11clonesize.html), determined by Pulsed Field Gel Electrophoresis analysis.

Format

Source BioScience offers the complete clone set of 1440 384-well microtitre plates or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.

How to Order

Due to MTA requirements, RPCI-11 clones and libraries should be ordered online using GenomeCube, using the clone ID format e.g. RP11-96I16 to search for individual clones.

 

We recommend using the public databases to find the RPCI-11 clone you are looking for, click here for advice on how to search for your clone.

 

Subsequent publication:

For any publications arising from the use of these clones please acknowledge the originators of the library, Kazutoyo Osoegawa, Minako Tateno and Pieter deJong, and Source BioScience for providing the clones in any publication arising out of using this resource.

References:

1. Osoegawa, K., Mammoser, A. G., Wu, C., Frengen, E., Zeng, C., Catanese, J. J., de Jong, P. J. (2001) A Bacterial Artificial Chromosome Library for Sequencing the Complete Human Genome, Genome Research, Vol. 11, Issue 3, 483-496, March 2001

2. Osoegawa, K., Woon, P.Y., Zhao, B., Frengen, E., Tateno, M., Catanese, J.J, and de Jong, P.J. (1998) An Improved Approach for Construction of Bacterial Artificial Chromosome Libraries, Genomics 52, 1-8; Article # GE985423.