Human Minimal genomic clone set

Minimal Genomic Clone Set

Human genomic clone set - Version 1.0

Description

Genomic Clones are a valuable resource for the functional analysis of genes, they can be used for a multitude of applications:

  • FISH - Fluorescent In Situ Hybridization - analysis of chromosomal rearrangements
  • Matrix-CGH Comparative Genomic Hybridization - high resolution analysis of genomic DNA
  • Protein - DNA binding studies - promotor, enhancer, silencer studies
  • Subcloning of control elements

How to find genomic clones of interest

You can search for genomic clones by:

  • Keywords or general IDs (like accession number, clone ID,...) using GenomeCUBE®. This search type is currently limited to Homo sapiens
  • Downloading the complete annotated clone set information as well as chromosomal subsets, listed below

Human Minimal Genomic Clone Set: Chromosome 1 - 3

Human Minimal Genomic Clone Set: Chromosome 4 - 6

Human Minimal Genomic Clone Set: Chromosome 7 - 9

Human Minimal Genomic Clone Set: Chromosome 10 - 12

Human Minimal Genomic Clone Set: Chromosome 13 - 15

Human Minimal Genomic Clone Set: Chromosome 16 - 18

Human Minimal Genomic Clone Set: Chromosome 19 - 21

Human Minimal Genomic Clone Set: Chromosome 22 - XY

Annotated Clone List

Clone selection criteria

The Clone Set was developed on basis of NCBI's genome assembly and the Ensembl annotation. The current clone set consists of 25,130 clones. The majority (22,898 clones, 91%) of which have been completely sequenced during the human genome sequencing project. Gaps were covered by 2,232 end-sequenced clones as far as such clones were available. We focused on publicly available BAC and PAC clones and completed the set where necessary with cosmids. The overall average insert size is approx 130 kb.

Product range

  • Clone Collection in microtiter plates
  • Customized subsets

Quality control

  • Viabilty
  • Contamination check (absence of contaminations of bacteriophages and non-E. coli microorganims)
  • Partly sequence-verified (end-sequencing of more than 7,400 clones)

References

Candidate Genes in Breast Cancer Revealed by Microarray-Based Comparative Genomic Hybridization of Archived Tissue
Cancer Research 65, 439-447, January 15, 2005

Detection of Chromosomal Imbalances in Retinoblastoma by Matrix-Based Comparative Genomic Hybridization
CANCER 43:294-301 (2005)