RPCI-23 (RP23) Mouse BAC vector

RPCI-23 (RP23) Mouse BAC clones (in E.coli)

 

Clones are streaked onto LB agar containing chloramphenicol (20µg/ml).



To use: restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.

 

Single colonies are then ready for DNA isolation, if required, using the accompanying protocol which although described for PAC clones also works for BAC clones.

Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.

 

mouse bac plasmid

 

1 gatccgcgga attcgagctc acgcgtactg atgcatgatc cgggtttaaa cccagtactc

61 tagatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg

1 gatccgcgga attcgagctc acgcgtactg atgcatgatc cgggtttaaa cccagtactc

61 tagatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt

121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa

181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac

241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg

301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc

361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat

421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca

481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc

541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc

601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg

661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta

721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg

781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac

841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag

901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat

961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat

1021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc

1081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt

1141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct

1201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt

1261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc

1321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac

1381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat

1441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg

1501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata

1561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta

1621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt

1681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag

1741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa

1801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc

1861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt

1921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc

1981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta

2041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa

2101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca

2161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac

2221 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta

2281 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt

2341 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc

2401 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt

2461 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc

2521 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat

2581 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc

2641 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt

2701 cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga tctagagtac

2761 tggttgaaac ccggatcatg catcagtacg cgtgagctcg aattccgcgg atccttctat

2821 agtgtcacct aaatgtcgac ggccaggcgg ccgccaggcc tacccactag tcaattcggg

2881 aggatcgaaa cggcagatcg caaaaaacag tacatacaga aggagacatg aacatgaaca

2941 tcaaaaaaat tgtaaaacaa gccacagttc tgacttttac gactgcactt ctggcaggag

3001 gagcgactca agccttcgcg aaagaaaata accaaaaagc atacaaagaa acgtacggcg

3061 tctctcatat tacacgccat gatatgctgc agatccctaa acagcagcaa aacgaaaaat

3121 accaagtgcc tcaattcgat caatcaacga ttaaaaatat tgagtctgca aaaggacttg

3181 atgtgtggga cagctggccg ctgcaaaacg ctgacggaac agtagctgaa tacaacggct

3241 atcacgttgt gtttgctctt gcgggaagcc cgaaagacgc tgatgacaca tcaatctaca

3301 tgttttatca aaaggtcggc gacaactcaa tcgacagctg gaaaaacgcg ggccgtgtct

3361 ttaaagacag cgataagttc gacgccaacg atccgatcct gaaagatcag acgcaagaat

3421 ggtccggttc tgcaaccttt acatctgacg gaaaaatccg tttattctac actgactatt

3481 ccggtaaaca ttacggcaaa caaagcctga caacagcgca ggtaaatgtg tcaaaatctg

3541 atgacacact caaaatcaac ggagtggaag atcacaaaac gatttttgac ggagacggaa

3601 aaacatatca gaacgttcag cagtttatcg atgaaggcaa ttatacatcc ggcgacaacc

3661 atacgctgag agaccctcac tacgttgaag acaaaggcca taaatacctt gtattcgaag

3721 ccaacacggg aacagaaaac ggataccaag gcgaagaatc tttatttaac aaagcgtact

3781 acggcggcgg cacgaacttc ttccgtaaag aaagccagaa gcttcagcag agcgctaaaa

3841 aacgcgatgc tgagttagcg aacggcgccc tcggtatcat agagttaaat aatgattaca

3901 cattgaaaaa agtaatgaag ccgctgatca cttcaaacac ggtaactgat gaaatcgagc

3961 gcgcgaatgt tttcaaaatg aacggcaaat ggtacttgtt cactgattca cgcggttcaa

4021 aaatgacgat cgatggtatt aactcaaacg atatttacat gcttggttat gtatcaaact

4081 ctttaaccgg cccttacaag ccgctgaaca aaacagggct tgtgctgcaa atgggtcttg

4141 atccaaacga tgtgacattc acttactctc acttcgcagt gccgcaagcc aaaggcaaca

4201 atgtggttat cacaagctac atgacaaaca gaggcttctt cgaggataaa aaggcaacat

4261 ttgcgccaag cttcttaatg aacatcaaag gcaataaaac atccgttgtc aaaaacagca

4321 tcctggagca aggacagctg acagtcaact aataacagca aaaagaaaat gccgatactt

4381 cattggcatt ttcttttatt tctcaacaag atggtgaatt gactagtggg tagatccaca

4441 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca

4501 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt

4561 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga

4621 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca

4681 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac

4741 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc

4801 aaacatgaga attgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag

4861 ttattaggtc cctcgactat agggtcaccg tcgacagcga cacacttgca tcggatgcag

4921 cccggttaac gtgccggcac ggcctgggta accaggtatt ttgtccacat aaccgtgcgc

4981 aaaatgttgt ggataagcag gacacagcag caatccacag caggcataca accgcacacc

5041 gaggttactc cgttctacag gttacgacga catgtcaata cttgcccttg acaggcattg

5101 atggaatcgt agtctcacgc tgatagtctg atcgacaata caagtgggac cgtggtccca

5161 gaccgataat cagaccgaca acacgagtgg gatcgtggtc ccagactaat aatcagaccg

5221 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc

 5281 gtggttccag actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata

5341 atcagaccga cgatacgagt gggaccatgg tcccagacta ataatcagac cgacgatacg

 5401 agtgggaccg tggtcccagt ctgattatca gaccgacgat acgagtggga ccgtggtccc

 5461 agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagactaa taatcagacc

5521 gacgatacga gtgggaccgt ggtcccagtc tgattatcag accgacgata caagtggaac

5581 agtgggccca gagagaatat tcaggccagt tatgctttct ggcctgtaac aaaggacatt

5641 aagtaaagac agataaacgt agactaaaac gtggtcgcat cagggtgctg gcttttcaag

5701 ttccttaaga atggcctcaa ttttctctat acactcagtt ggaacacgag acctgtccag

5761 gttaagcacc attttatcgc ccttatacaa tactgtcgct ccaggagcaa actgatgtcg

5821 tgagcttaaa ctagttcttg atgcagatga cgttttaagc acagaagtta aaagagtgat

5881 aacttcttca gcttcaaata tcaccccagc ttttttctgc tcatgaaggt tagatgcctg

5941 ctgcttaagt aattcctctt tatctgtaaa ggctttttga agtgcatcac ctgaccgggc

6001 agatagttca ccggggtgag aaaaaagagc aacaactgat ttaggcaatt tggcggtgtt

6061 gatacagcgg gtaataatct tacgtgaaat attttccgca tcagccagcg cagaaatatt

6121 tccagcaaat tcattctgca atcggcttgc ataacgctga ccacgttcat aagcacttgt

6181 tgggcgataa tcgttaccca atctggataa tgcagccatc tgctcatcat ccagctcgcc

6241 aaccagaaca cgataatcac tttcggtaag tgcagcagct ttacgacggc gactcccatc

6301 ggcaatttct atgacaccag atactcttcg accgaacgcc ggtgtctgtt gaccagtcag

6361 tagaaaagaa gggatgagat catccagtgc gtcctcagta agcagctcct ggtcacgttc

6421 attacctgac catacccgag aggtcttctc aacactatca ccccggagca cttcaagagt

6481 aaacttcaca tcccgaccac atacaggcaa agtaatggca ttaccgcgag ccattactcc

6541 tacgcgcgca attaacgaat ccaccatcgg ggcagctggt gtcgataacg aagtatcttc

6601 aaccggttga gtattgagcg tatgttttgg aataacaggc gcacgcttca ttatctaatc

6661 tcccagcgtg gtttaatcag acgatcgaaa atttcattgc agacaggttc ccaaatagaa

6721 agagcatttc tccaggcacc agttgaagag cgttgatcaa tggcctgttc aaaaacagtt

6781 ctcatccgga tctgaccttt accaacttca tccgtttcac gtacaacatt ttttagaacc

6841 atgcttcccc aggcatcccg aatttgctcc tccatccacg gggactgaga gccattacta

6901 ttgctgtatt tggtaagcaa aatacgtaca tcaggctcga accctttaag atcaacgttc

6961 ttgagcagat cacgaagcat atcgaaaaac tgcagtgcgg aggtgtagtc aaacaactca

7021 gcaggcgtgg gaacaatcag cacatcagca gcacatacga cattaatcgt gccgataccc

7081 aggttaggcg cgctgtcaat aactatgaca tcatagtcat gagcaacagt ttcaatggcc

7141 agtcggagca tcaggtgtgg atcggtgggc agtttacctt catcaaattt gcccattaac

7201 tcagtttcaa tacggtgcag agccagacag gaaggaataa tgtcaagccc cggccagcaa

7261 gtgggcttta ttgcataagt gacatcgtcc ttttccccaa gatagaaagg caggagagtg

7321 tcttctgcat gaatatgaag atctggtacc catccgtgat acattgaggc tgttccctgg

7381 gggtcgttac cttccacgag caaaacacgt agccccttca gagccagatc ctgagcaaga

7441 tgaacagaaa ctgaggtttt gtaaacgcca cctttatggg cagcaacccc gatcaccggt

7501 ggaaatacgt cttcagcacg tcgcaatcgc gtaccaaaca catcacgcat atgattaatt

7561 tgttcaattg tataaccaac acgttgctca acccgtcctc gaatttccat atccgggtgc

7621 ggtagtcgcc ctgctttctc ggcatctctg atagcctgag aagaaacccc aactaaatcc

7681 gctgcttcac ctattctcca gcgccgggtt attttcctcg cttccgggct gtcatcatta

7741 aactgtgcaa tggcgatagc cttcgtcatt tcatgaccag cgtttatgca ctggttaagt

7801 gtttccatga gtttcattct gaacatcctt taatcattgc tttgcgtttt tttattaaat

7861 cttgcaattt actgcaaagc aacaacaaaa tcgcaaagtc atcaaaaaac cgcaaagttg

7921 tttaaaataa gagcaacact acaaaaggag ataagaagag cacatacctc agtcacttat

7981 tatcactagc gctcgccgca gccgtgtaac cgagcatagc gagcgaactg gcgaggaagc

8041 aaagaagaac tgttctgtca gatagctctt acgctcagcg caagaagaaa tatccaccgt

8101 gggaaaaact ccaggtagag gtacacacgc ggatagccaa ttcagagtaa taaactgtga

8161 taatcaaccc tcatcaatga tgacgaacta acccccgata tcaggtcaca tgacgaaggg

8221 aaagagaagg aaatcaactg tgacaaactg ccctcaaatt tggcttcctt aaaaattaca

8281 gttcaaaaag tatgagaaaa tccatgcagg ctgaaggaaa cagcaaaact gtgacaaatt

8341 accctcagta ggtcagaaca aatgtgacga accaccctca aatctgtgac agataaccct

8401 cagactatcc tgtcgtcatg gaagtgatat cgcggaagga aaatacgata tgagtcgtct

8461 ggcggccttt ctttttctca atgtatgaga ggcgcattgg agttctgctg ttgatctcat

8521 taacacagac ctgcaggaag cggcggcgga agtcaggcat acgctggtaa ctttgaggca

8581 gctggtaacg ctctatgatc cagtcgattt tcagagagac gatgcctgag ccatccggct

8641 tacgatactg acacagggat tcgtataaac gcatggcata cggattggtg atttcttttg

8701 tttcactaag ccgaaactgc gtaaaccggt tctgtaaccc gataaagaag ggaatgagat

8761 atgggttgat atgtacactg taaagccctc tggatggact gtgcgcacgt ttgataaacc

8821 aaggaaaaga ttcatagcct ttttcatcgc cggcatcctc ttcagggcga taaaaaacca

8881 cttccttccc cgcgaaactc ttcaatgcct gccgtatatc cttactggct tccgcagagg

8941 tcaatccgaa tatttcagca tatttagcaa catggatctc gcagataccg tcatgttcct

9001 gtagggtgcc atcagatttt ctgatctggt caacgaacag atacagcata cgtttttgat

9061 cccgggagag actatatgcc gcctcagtga ggtcgtttga ctggacgatt cgcgggctat

9121 ttttacgttt cttgtgattg ataaccgctg tttccgccat gacagatcca tgtgaagtgt

9181 gacaagtttt tagattgtca cactaaataa aaaagagtca ataagcaggg ataactttgt

9241 gaaaaaacag cttcttctga gggcaatttg tcacagggtt aagggcaatt tgtcacagac

9301 aggactgtca tttgagggtg atttgtcaca ctgaaagggc aatttgtcac aacaccttct

9361 ctagaaccag catggataaa ggcctacaag gcgctctaaa aaagaagatc taaaaactat

9421 aaaaaaaata attataaaaa tatccccgtg gataagtgga taaccccaag ggaagttttt

9481 tcaggcatcg tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc ctcgctcact

9541 cgagggcttc gccctgtcgc tcaactgcgg cgagcactac tggctgtaaa aggacagacc

9601 acatcatggt tctgtgttca ttaggttgtt ctgtccattg ctgacataat ccgctccact

9661 tcaacgtaac accgcacgaa gatttctatt gttcctgaag gcatattcaa atcgttttcg

9721 ttaccgcttg caggcatcat gacagaacac tacttcctat aaacgctaca caggctcctg

9781 agattaataa tgcggatctc tacgataatg ggagattttc ccgactgttt cgttcgcttc

9841 tcagtggata acagccagct tctctgttta acagacaaaa acagcatatc cactcagttc

9901 cacatttcca tataaaggcc aaggcattta ttctcaggat aattgtttca gcatcgcaac

9961 cgcatcagac tccggcatcg caaactgcac ccggtgccgg gcagccacat ccagcgcaaa

10021 aaccttcgtg tagacttccg ttgaactgat ggacttatgt cccatcaggc tttgcagaac

10081 tttcagcggt ataccggcat acagcatgtg catcgcatag gaatggcgga acgtatgtgg

10141 tgtgaccgga acagagaacg tcacaccgtc agcagcagcg gcggcaaccg cctccccaat

10201 ccaggtcctg accgttctgt ccgtcacttc ccagatccgc gctttctctg tccttcctgt

10261 gcgacggtta cgccgctcca tgagcttatc gcgaataaat acctgtgacg gaagatcact

10321 tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt

10381 ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa

10441 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa

10501 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac

10561 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata

10621 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc

10681 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg

10741 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa

10801 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt

10861 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga

10921 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg

10981 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg

11041 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg

11101 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat

11161 ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata

11221 ataagcggat gaatggcaga aattcgatga taagctgtca aacatgagaa ttggtcgacg

11281 gcgcgccaaa gcttgcatgc ctgcagccgc gtaacctggc aaaatcggtt acggttgagt

11341 aataaatgga tgccctgcgt aagcggggca catttcatta cctctttctc cgcacccgac

11401 atagataata acttcgtata gtatacatta tacgaagtta tctagtagac ttaattaagg

11461 atcgatccgg cgcgccaata gtcatgcccc gcgcccaccg gaaggagctg actgggttga

11521 aggctctcaa gggcatcggt cgagcttgac attgtaggac tatattgctc taataaattt

11581 gcggccgcta atacgactca ctatagggag ag