Clones are streaked onto LB agar containing kanamycin (25µg/ml).
To use: restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.
Single colonies are then ready for DNA isolation, if required, using the accompanying protocol.
Clones can be grown in LB broth + kanamycin, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.
Further information on the vector can be obtained from http://bacpac.chori.org/vectorsdet.htm
Please acknowledge the originator of the library, Pieter de Jong , and Source BioScience LifeSciences for providing the clone in any publication arising out of using this resource. An example of how a clone (E.g. 471-m19) should be described in a publication or database entry is: RP21-471M19 from the RPCI mouse PAC library 21.