Prior to ordering this library we strongly recommend that you read the following publication:
Contents of package:
Specific product information:
The following PCR cycle has been successfully used for amplification of the CpG island fragments. We strongly recommend that the conditions are optimized, to allow for lab to lab variation.
5' -TAA TAC GAC TCA CTA TAG GG- 3' (T7) ; 5' -GAT TTA GGT GAC ACT ATA G- 3' (SP6)
Purification of CpG islands using a methylated DNA binding column Cross SH, Charlton JA, Nan X and Bird AP. Nature Genetics, 1994 6 (3), 236-244