Contents of package:
Specific product information:
5'-CGG CCG CCT GCA GGT CGA CCT TAA- 3' (Source BioScience LifeSciences no 3558) 5'-AAC GCG TTG GGA GCT CTC CCT TAA-3' (Source BioScience LifeSciences no 3559)
Additional primer stocks can be obtained through Source BioScience LifeSciences quoting the above reference numbers.
The following PCR cycle has been successfully used for amplification of the CpG island fragments. We strongly recommend that the conditions are optimized, to allow for lab to lab variation.
5' -TAA TAC GAC TCA CTA TAG GG- 3' (T7) ; 5' -GAT TTA GGT GAC ACT ATA G- 3' (SP6)
Cross SH, Lee M, Clark VH, Craig JM, Bird AP and Bickmore WA. The Chromosmal Distribution of CpG islands in the Mouse: Evidence for Genome Scrambling in the Rodent Lineage Genomics 1997 40 454-457