Human TilePath library (clones and plates)


Please note that the clone(s) being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.

THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.

What to do with your clones when you receive them

Plates contain LB broth with 8% glycerol and the appropriate antibiotic. The plates are sent on Dry Ice, and should be stored at -70°C

Library name Plate numbers available Antibiotic
RPCI1 1-321 25μl/ml Kanamycin
RPCI3 322-528 25μl/ml Kanamycin
RPCI4 529-816 25μl/ml Kanamycin
RPCI5 817-1200 25μl/ml Kanamycin
RPCI6 1-240 25μl/ml Kanamycin
RPCI11 1-576 20μl/ml Chloramphenicol
RPCI13 1-480 20μl/ml Chloramphenicol

 

Individual clones have been streaked onto LB agar containing the appropriate antibiotic. Please store them at 4°C (not in a freezer). As soon as possible, transfer the culture to LB broth containing the antibiotic + 8% glycerol and incubate overnight at 37°C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37°C for subsequent freezing at -70°C.

These clones are for research purposes only.

Acknowledgements and subsequent publication:

Osoegawa K, Mammoser AG, Wu C, Frengen E, Zeng C, Catanese JJ, de Jong PJ. A bacterial artificial chromosome library for sequencing the complete human genome. Genome Res. 2001 Mar;11(3):483-96.

The Wellcome Trust Sanger Institute, Hixton Campus, Cambridge and Roswell Park Cancer Institute, Buffalo, New York.