Mouse bMQ BAC library

We are pleased to announce the distribution of a fully indexed mouse BAC library, constructed in Alan Bradley′s lab, The Wellcome Trust Sanger Institute, Hinxton, UK.

Library specifications

The library was generated from AB2.2 ES cell DNA (129S7/SvEv Brd-Hprt b-m2) and represents 3.6 autosome and 1.24 sex chromosome coverage, with an average insert size of 110.68 kb. The clones cover over 97% of the mouse genome and 99.1 % of Ensembl genes. Finally, the BAC′s have been end-sequence profiled using SP6 and T7 primers, giving an average read-ends size of 739bp.

129s7/AB2.2 BAC clones are displayed on the Ensembl genome browser within a DAS (Distributed Annotation Server). The clones are displayed as green (the T7 reads from the telomere to the centromere) and pink (the T7 reads from centromere to telomere ), which indicates the orientation of the DNA insert in the vector, whilst end reads, are shown as grey bars.

A library of this quality is ideal for the construction of gene targeting vectors. It may also be a useful tool for examining large-scale structural differences between 129v and other mouse strain genomes and facilitating high-throughput targeted manipulation of the mouse genome.

Format

We offer the complete clone set of 434 384-well microtitre plates or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.

How to Order

Due to MTA requirements, bMQ clones and libraries should be ordered online using GenomeCube, using the clone ID format e.g. BMQ-38M6 to search for individual clones.

 

Reference

A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction. Adams DJ, Quail MA, Cox T, van der Weyden L, Gorick BD, Su Q, Chan WI, Davies R, Bonfield JK, Law F, Humphray S, Plumb B, Liu P, Rogers J, Bradley A. Genomics. 2005 Dec;86(6):753-8. Epub 2005 Oct 27. The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, UK.