bMQ Mouse BAC library storage and handling


Please note that the clone(s) being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.

THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.

What to do with your clones when you receive them

Plates contain LB broth with 7.5% glycerol and chloramphenicol. The plates are sent on Dry Ice, and should be stored at -70°C.

Individual clones pools have been streaked onto LB agar containing chloramphenicol (20 μg/ml). Please store them at 4°C (not in a freezer). As with all clones, it is recommended that you re-streak to obtain several single colonies to test. As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37ºC.

A selection of at least 10 single colonies should be picked and grown overnight at 37ºC in LB broth containing chloramphemicol + 8% glycerol for subsequent freezing at -70 ºC. These frozen stocks can then be re-grown for plasmid isolation and purification.  

 

These clones are for research purposes only.

 

bMQ library, E.coli host strain

 

Name: DH10B

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG