CORF Library (clones and plates)


Please note that the clone(s) being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.

THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.

What to do with your clones when you receive them

Plates contain LB broth with 7.5 % glycerol and spectinomycin. These are sent on Dry Ice. These should be stored at -70°C

Individual clones pools have been streaked onto LB agar containing spectinomycin (100 μg/ml). Please store them at 4°C (not in a freezer). As soon as possible, transfer the culture to LB broth containing spectinomycin + 7.5 % glycerol and incubate overnight at 37°C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37°C for subsequent freezing at -70°C.

Bacterial strain Mach1 (InVitrogen plc) Genotype: F - φ80(lacZ)ΔM15 ΔlacX74 hsdR(rk-mk+) ΔrecA1398 endA1 tonA

These clones are for research purposes only.