The Gateway® Technology of Invitrogen Inc. is a universal cloning system that takes advantage of the site-specific recombination properties of bacteriophage lambda (Landy, 1989) to provide a rapid and highly efficient way to move your gene of interest into multiple vector systems.
We shuttle all ORFs into the following Gateway® expression (destination) vectors on demand and provide ready-to-express Full ORF Expression Clones.
Please take care not to combine a clone with stopcodon and a vector with C-terminal tags, since they are not expressed.
Expression vector (Gateway®)
Selection in expression host
Please note, that the region between 'attR1' and 'attR2' in the vector maps (including i.e. Chloramphenicol resistance, cleaving sites) is replaced by the ORF.
For other Gateway® expression vectors please contact customer services.