ORF shuttle clones – details


Full ORF Clone Benefits

  • Flexible Gateway® cloning system
  • Insert fully sequenced at high accuracy (no frame shift, no stopcodon in frame, error rate <1/10000)
  • Sequences publicly available (EMBL, GenBank, DDBJ)
  • Amino acid exchanges compared to reference sequence annotated, if applicable
  • Sequences upstream of ATG and downstream of stopcodon annotated

Gateway® Expression Shuttle System

The Gateway® technology of Invitrogen Inc. is a universal cloning system that takes advantage of the site-specific recombination properties of bacteriophage lambda (Landy, 1989) to provide a rapid and highly efficient way to move your gene of interest into multiple vector systems.

 You may get the desired Gateway® expression (destination) vectors at www.invitrogen.com.

 

Available expression vectors

Protein expression in E. coli

  • pBAD-DEST49: N-term. Thioredoxin (with enterokinase recogn. site), C-term. V5 and His6 tag
  • pDEST14
  • pDEST15: N-terminal GST tag
  • pDEST17: N-terminal His6 tag
  • pDEST17-D18: N-terminal His6 tag
  • pDEST24: C-terminal GST tag
  • pDEST42: C-terminal V5 and His6 tag
  • pET104-DEST: C-term. BioEase (for in vivo biotinylation) and Xpress tag (with enterokinase recogn. site)
  • pEXP1-DEST: N-term. His6 and Xpress tag (with enterokinase recogn. site)
  • pEXP2-DEST: C-terminal V5 and His6 tag

Protein expression in yeast

  • pDEST22: Y2H activation domain vector, N-term. GAL4 domain
  • pDEST32: Y2H DNA binding domain vector, N-term. GAL4 domain
  • pYES-DEST52: C-terminal V5 and His6 tag
  • pEXP-AD502: Y2H activation domain vector, N-term. GAL4 domain with NLS

Protein expression in baculovirus

  • pDEST8: native, without tag
  • pDEST10: N-term. His6 tag with TEV protease recogn. site
  • pDEST20: N-term. GST tag
  • pMT_BioEase-DEST: N-term. BioEase tag for in vivo biotinylation with enterokinase recogn. site
  • pMT-DEST48: C-terminal V5 and His6 tag

Protein expression in mammalian cells

  • pAdCMV_V5-DEST: C-terminal V5 tag
  • pAdPL-DEST: native, promotor of choice
  • pcDNA-DEST40: C-terminal V5 and His6 tag
  • pcDNA-DEST47: C-terminal cycle 3 GFP tag
  • pcDNA-DEST53: N-terminal cycle 3 GFP tag
  • pcDNA3.1-nV5-DEST: N-term. V5 tag (with TEV protease recogn. site)
  • pcDNA3.2-V5-DEST: C-terminal V5 tag
  • pcDNA6.2-V5-DEST: C-terminal V5 tag
  • pEF5FRT_V5-DEST: C-terminal V5 tag
  • pEF-DEST51: C-terminal V5 and His6 tag
  • pLenti6V5-DEST: C-terminal V5 tag
  • pT-REx-DEST30: tet inducible
  • pT-REx-DEST31: N-terminal His6 tag, tet inducible



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