The vector backgrounds used for the Structural Genomic Consortium clones are also available to purchase through Source BioScience.

Vectors are supplied as E.coli stocks streaked onto an agar slope containing the appropriate selective antibiotic.  Click on the Vector Name link in the table below to find further information on each vector.

The price of the following vectors is £45 each (excluding VAT and delivery charges). For information on how to order see below.

Vector Name

Description

Antibiotic resistance

pNIC28-Bsa4

pET expression vector with His6 tag in 22-aa N-terminal fusion peptide, with TEV protease cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection on 5% sucrose

Kanamycin, 50 μg/ml

pNIC-ZB

 

pET expression vector with His6 and Z-basic (Zb) tags followed by a TEV protease cleavage site. The Z-basic tag (J. Chromatog A, 1161:22-28) is a 54-aa sequence derived from protein A and modified to have a high positive surface charge, allowing the fusion proteins to bind to S-sepharose at salt concentrations in which most cellular proteins do not bind. The vector includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection on 5% sucrose

Kanamycin, 50 μg/ml

pNIC-CTHF

pET expression vector with C-termina His6 tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection on 5% sucrose

Kanamycin, 50 μg/ml

pNIC-CH

 

pET expression vector with C-terminal His6 tag. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection on 5% sucrose

Kanamycin, 50 μg/ml

pNH-TrxT

pET expression vector with His6-Trx (E. coli thioredoxin) in 128-aa N-terminal fusion peptide, with TEV protease cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection on 5% sucrose

Kanamycin, 50 μg/ml

pBEN1-SGC

pET expression vector with a 94-aa N-terminal fusion derived from pBEn-SBP-SET1a (Stratagene) followed by a TEV protease cleavage site. This tag ellows purification on streptavidine columns, and may enhance solubility of fusion proteins. The vector includes sites for LIC cloning, and a "stuffer"  fragment that includes the SacB gene, allowing negative selection on 5% sucrose.

Kanamycin, 50 μg/ml

pTvHR21-SGC

pET21a-derived expression vector with C-terminal His6 tag, removable with TEV protease. A "stuffer" fragment that includes the SacB gene, allows negative selection on 5% sucrose for indigested vector.

Ampicillin

pLIC-SGC1

pET expression vector with His6 tag in 23-aa N-terminal fusion peptide, with TEV protease cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection on 5% sucrose

Ampicillin

pGTvL1-SGC

pGEX expression vector with N-terminal GST tag and TEV protease cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection on 5% sucrose.

ampicillin

pFB-Sec-NH

 

Baculovirus transfer vector with a signal peptide (from baculovirus gp64), a His 6 tag and a TEV protease cleavage site fused to the N-terminal of the cloned protein. Used for secreted or integral membrane proteins. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection of transformed bacteria on 5% sucrose

Ampicillin, 100 μg/ml

pFB-LIC-Bse

Baculovirus transfer vector with His6 tag in 22-aa N-terminal fusion peptide, with TEV protease cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection of transformed bacteria on 5% sucrose

Ampicillin, 100 μg/ml

pFB-CT10HF-LIC

Baculovirus transfer vector with with C-terminal His10 tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, allowing negative selection on 5% sucrose

Ampicillin, 100 μg/ml

 

How to order

The SGC expression clone background vectors are not currently searchable via our GenomeCUBE® search and cannot be ordered online.  We are in the process of integrating the data, however in the meantime you can still order these clones using a purchase order sent by email or fax to our customer services at the contact details below.

1.  Use the Vector ID to place your purchase order.

Price per vector £45

(Prices exclude VAT and shipping)

2. Print off, read and sign the appropriate Material Transfer Agreement:

US-only

non-US

3. Send your order and signed Material Transfer Agreement to:

Fax: +44 (0) 115 973 9021

e-mail: sales@sourcebioscience.com

References

Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown NA, Gileadi O. High-throughput production of human proteins for crystallization: the SGC experience. J Struct Biol. 172(1):3-13. (2010)

 

Gileadi O, Knapp S, Lee WH, Marsden BD, Muller S, Niesen FH, Kavanagh KL, Ball LJ, von Delft F, Doyle DA, Oppermann UC, Sundstrom M. The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins J Struct Funct Genomics 15(10):1215-26 (2007)