Please note that the clone(s) being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.
THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.
What to do with your clones when you receive them
Plates contain LB broth with 8% glycerol and ampicillin. The plates are sent on Dry Ice, and should be stored at -70 °C
Individual clones have been streaked onto LB agar containing ampicillin (100 µg/ml). Please store them at 4 °C (not in a freezer). As soon as possible, transfer the culture to LB broth containing ampicillin + 8% glycerol and incubate overnight at 37 °C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37 °C for subsequent freezing at -70 °C.
These clones are for research purposes only.
Acknowledgements and subsequent publication
Please acknowledge the Netherlands Cancer Institute (NKI) and Source BioScience LifeSciences in any resulting publications.
Please also cite the following paper:
Voorhoeve PM, le Sage C, Schrier M, Gillis AJM, Stoop H, Nagel R, Liu YP, van Duijse Y, Drost J, Griekspoor A, Zlotorynski E, Yabuta N, De Vita G,Nojima H, Looijenga, LHJ and Agami R.
A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors.
Cell. 2006 Mar 24;124(6):1169-81.