C. elegans ORF-RNAi Library v1.1

This ORF-based RNAi library is ideal for either high-throughput screening or individual knockdown experiments.

The ORFeome-RNAi v1.1 library contains 11,511 RNAi clones, which targets 10,953; 485 of these genes are targeted by 2 or more constructs. The library therefore covers the potential knockdown of 55% of the 19,920 unique protein-coding genes predicted in "worm sequence" 112.

Interestingly, there are ~1,700 unique genes not previously targeted by existing RNAi feeding libraries (an additional ~9% of the worm genome). Therefore, in conjunction with our exciting RNAi feeding library he overall predicted coverage of worm genes is ~86%.

Format:

 
Source BioScience LifeSciences offers individual clone(s) for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.

Reference:

 
Rual JF, Ceron J, Koreth J, Hao T, Nicot AS, Hirozane-Kishikawa T, Vandenhaute J, Orkin SH, Hill DE, van den Heuvel S, Vidal M. "Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library". Genome Res. 2004 Oct;14(10B):2162-8. Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.