C. elegans RNAi library
This Caenorhabditis elegans RNAi feeding library
provided by Source BioScience LifeSciences was constructed by Julie
Ahringer's group at the The Wellcome CRC Institute, University of
Cambridge, Cambridge, England.
C. elegans genomic fragments
were PCR amplified using Research Genetics GenePairs, cloned into
the EcoRV site of vector L4440 from Timmons and Fire ( Nature, 395, 854) and transformed into
bacterial strain HT115 ( Gene, 263, 103-112) as described ( Nature 408, 325-330). The whole genome library
consists of 16,757 bacterial strains, which cover 87% of C.
elegans genes.
Bacterial strains carry the GenePairs
name, which usually, but does not always correspond to a predicted
C. elegans gene name. A current mapping of GenePair to
gene can be found in WormBase (http://www.wormbase.org/). GenePairs primer
sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.
There is also a "C.elegans
Finder" tool available which allows you to find the primer
sequences for any given GenePair Name in addition to the location
of that bacterial strain in Source BioScience LifeSciences 384-well
plates. The complete C.elegans RNAi database can be
downloaded here . Note that the mapping of
some GenePairs has changed since WS56 when this database was
built.
The library is available by individual chromosome sets (I, II, III,
IV, V and X) from Source BioScience LifeSciences as frozen glycerol
stocks of bacterial strains arrayed in 384 well plates or as
individual bacterial strains (clones).
We are also supplying re-arrayed
sub-sets of the libraries in the following areas:
- Chromatin (257 clones)
- Phosphatase (166 clones)
- Transcription factors (387 clones).