C. elegans RNAi library
Product Description
The Caenorhabditis elegans RNAi feeding library was
constructed by Julie Ahringer's group at The Wellcome CRC
Institute, University of Cambridge, Cambridge, UK and is
distributed by Source BioScience LifeSciences. It is designed
for genome wide study of gene function in C. elegans
through loss of function studies. It can be used both for
rapid large scale RNAi experiments and for the study of
individual genes.
When produced initially the library targeted 72% of
C.elegans genes (according to current gene
annotation). Julie Ahringer and her team in conjuction
with Source BioScience have since generated a collection
of new supplemental bacterial strains to enhance this library, and
the total library now targets around 87% of currently annotated
C.elegans genes. These supplemental strains are now
available to order from Source BioScience LifeSciences.
The library is available as individual chromosome sets (I, II,
III, IV, V and X) which include the new
supplemental strains.
The complete supplemental RNAi set of plates
and individual chromosome sets (I, II, III, IV, V and X)
are also available to order for those who have already
purchased the library in the past.
Set Details
|
Library sets
|
Number of plates
|
Approximate clone number
|
|
C.elegans Chr.I RNAi
|
10
|
2,875
|
|
C.elegans Chr.II RNAi
|
11
|
3,594
|
|
C.elegans Chr.III RNAi
|
8
|
2,630
|
|
C.elegans Chr.IV RNAi
|
10
|
3,221
|
|
C.elegans Chr.V RNAi
|
16
|
4,716
|
|
C.elegans Chr.X RNAi
|
8
|
2,726
|
| C.elegans
Chr RNAi - complete set |
64 |
19,762 |
The complete C.elegans RNAi database including the
new supplementary bacterial strains can be
downloaded here.
|
NEW Supplementary sets
|
Number of plates
|
Approximate clone number
|
|
C.elegans supplementary Chr. RNAi - Chr.I
|
2
|
430
|
|
C.elegans supplementary Chr. RNAi - Chr.II
|
2
|
620
|
|
C.elegans supplementary Chr. RNAi - Chr.III
|
2
|
498
|
|
C.elegans supplementary Chr. RNAi - Chr.IV
|
2
|
528
|
|
C.elegans supplementary Chr. RNAi - Chr.V
|
3
|
1,060
|
|
C.elegans supplementary Chr. RNAi - Chr.X
|
1
|
371
|
|
C.elegans supplementary Chr. RNAi complete set
|
12
|
3,507
|
The library is provided as frozen glycerol stocks of bacterial
strains arrayed in 384 well plates.
Individual bacterial strains (clones) are also available and can
be found and ordered on GenomeCube. Individual strains
are available in sequence
verified and purified plasmid
formats.
Library details
C. elegans genomic fragments were PCR amplified, cloned
into the EcoRV site of vector L4440 from Timmons and Fire ( Nature, 395, 854) and transformed into
bacterial strain HT115 ( Gene, 263, 103-112) as described ( Nature 408, 325-330).
Further
details and feeding protocol.
For library and clone handling guidelines click
here
To find the location of the bacterial strain in Source
BioScience LifeSciences 384-well plates please use the database
files below:
The complete C.elegans RNAi database including the
new supplementary bacterial strains can be
downloaded here.
Note that the mapping of some primer pairs has
changed since these databases were built.
A current mapping of primer pairs to genes can be
found in C.elegans bioinformatics database WormBase by
searching using gene name or primer pair name (http://www.wormbase.org/) and the
primer pair sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.
We also supply re-arrayed sub-sets of
the libraries in the following areas:
-
Chromatin (257 clones)
-
Phosphatase (166 clones)
-
Transcription factors (387 clones).
How to order
Library, plate set and individual strain orders must be placed
via our website. An MTA must be agreed to during the on-line
ordering process and you can read this MTAs at the links below
Library of Bacterial
Strains of C. elegans genes (academic non-USA)
Library of Bacterial
Strains of C. elegans genes (academic USA)
Library of Bacterial
Strains of C. elegans genes (commercial non UK)
Library of Bacterial
Strains of C. elegans genes (commercial UK only)
To order the library and individual chromosome sets use our Quick Buy tool. Please note that
Individual bacterial strains can be found on our GenomeCube search using their primer
pair name. Click
here for more information.
Any questions?
Please contact our technical
team who will be happy to help you.