Human shRNAi Library
To complement Source BioScience LifeSciences′s™ expanding
portfolio of resources, a short-hairpin RNA (shRNA) library has
been constructed by the ′The Netherlands Cancer Institute′ (NKI).
This library has been tailored specifically for applications in
mammalian cancer genetics.
This library has substantial advantages over many
similar resources including full sequence verification and
knockdown validation.
Applications
The shRNA constructs have been designed and cloned into a
retroviral vector and can easily be utilised for gene silencing
experiments. Multiple shRNA expression vectors represent many of
the genes, each with at least one shRNA construct covering a unique
region of the target gene. Researchers can study the function of
specific genes and also search the human genome for novel
therapeutic targets for many diseases.
To search for knockdown shRNA candidates for your gene
of interest, please input your gene symbol:
Search & order
your clones now
Description
This library is based on the production of a shRNA expressed
from a vector harbouring the RNA polymerase III-driven promoter for
H1 RNA, the RNA component of human RNase P. The shRNA consist of a
target transcript-specific 19-base double stranded stem, connected
by a 9-base loop sequence.
- Library consists of 13,500 clones
- Targets ~5000 cancer related genes
- Controlled quantities of silencing reagent delivered to the
cell
- Up to 3 19-mer target sequence constructs per gene
- Backbone plasmid allows for the shRNA cassettes to be packaged
in retroviruses
- Unique barcode to expedite screening in high-throughput
experiments
Specifications
Each clone has the following:
- pRSC vector contains an H1 promoter
- Unique hairpin-loop knockdown cassette
- Two bacterial resistance markers (chloramphenicol &
ampicillin)
- Puromycin-selectable marker for mammalian cells
- H1 promoter + shRNA insert can be shuttled between different
vectors using EcoRI & XhoI sites
- Up to 3 19-mer target sequence constructs per gene
- Glycerols are ready for distribution in single clone and
library formats
Source BioScience LifeSciences offers the complete clone set of
36 384-well microtitre plate or individual clones, for research
purposes only. We can also make subsets of your chosen clones from
the collection. Please contact us
to discuss your requirements