pRetroSuperCam vector
Clones are streaked onto LB agar containing ampicillin (50µg/ml)
and chloramphenicol (20µg/ml). To use: re-streak the clone onto the
same agar type, in such a way as to obtain single colonies.
Incubate at 37°C overnight. Clones can be grown in LB broth +
ampicillin & chloramphenicol, and glycerol added after growth
to a concentration of 25% for long-term storage at -70°C.
1 tgaaagaccc cacctgtagg
tttggcaagc tagcttaagt aacgccattt tgcaaggcat 60
61 ggaaaataca taactgagaa tagagaagtt
cagatcaagg ttaggaacag agagacagca 120
121 gaatatgggc caaacaggat atctgtggta
agcagttcct gccccggctc agggccaaga 180
181 acagatggtc cccagatgcg gtcccgccct
cagcagtttc tagagaacca tcagatgttt 240
241 ccagggtgcc ccaaggacct gaaatgaccc
tgtgccttat ttgaactaac caatcagttc 300
301 gcttctcgct tctgttcgcg cgcttctgct
ccccgagctc aataaaagag cccacaaccc 360
361 ctcactcggc gcgccagtcc tccgatagac
tgcgtcgccc gggtacccgt attcccaata 420
421 aagcctcttg ctgtttgcat ccgaatcgtg
gactcgctga tccttgggag ggtctcctca 480
481 gattgattga ctgcccacct cgggggtctt
tcatttggag gttccaccga gatttggaga 540
541 cccctgccca gggaccaccg acccccccgc
cgggaggtaa gctggccagc ggtcgtttcg 600
601 tgtctgtctc tgtctttgtg cgtgtttgtg
ccggcatcta atgtttgcgc ctgcgtctgt 660
661 actagttagc taactagctc tgtatctggc
ggacccgtgg tggaactgac gagttctgaa 720
721 cacccggccg caaccctggg agacgtccca
gggactttgg gggccgtttt tgtggcccga 780
781 cctgaggaag ggagtcgatg tggaatccga
ccccgtcagg atatgtggtt ctggtaggag 840
841 acgagaacct aaaacagttc ccgcctccgt
ctgaattttt gctttcggtt tggaaccgaa 900
901 gccgcgcgtc ttgtctgctg cagcgctgca
gcatcgttct gtgttgtctc tgtctgactg 960
961 tgtttctgta tttgtctgaa aattagggcc
agactgttac cactccctta agtttgacct 1020
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca
gtcggtagat gtcaagaaga 1080
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt
taacgtcgga tggccgcgag 1140
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat
caaggtcttt tcacctggcc 1200
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg
ggaagccttg gcttttgacc 1260
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc
gcctcctctt cctccatccg 1320
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc
tcgatcctcc ctttatccag 1380
1381 ccctcactcc ttctctaggc gccggaatta gatccgttct
aacgtcgacg taccaattac 1440
1441 gccccgccct gccactcatc gcagtactgt tgtaattcat
taagcattct gccgacatgg 1500
1501 aagccatcac aaacggcatg atgaacctga atcgccagcg
gcatcagcac cttgtcgcct 1560
1561 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
agttgtccat attggccacg 1620
1621 tttaaatcaa aactggtgaa actcacccag ggattggctg
agacgaaaaa catattctca 1680
1681 ataaaccctt tagggaaata ggccaggttt tcaccgtaac
acgccacatc ttgcgaatat 1740
1741 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
agagcgatga aaacgtttca 1800
1801 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
cccatatcac cagctcaccg 1860
1861 tctttcattg ccatacggaa ctccggatga gcattcatca
ggcgggcaag aatgtgaata 1920
1921 aaggccggat aaaacttgtg cttatttttc tttacggtct
ttaaaaaggc cgtaatatcc 1980
1981 agctgaacgg tctggttata ggtacattga gcaactgact
gaaatgcctc aaaatgttct 2040
2041 ttacgatgcc attgggatat atcaacggtg gtatatccag
tgattttttt ctccatttta 2100
2101 gcttccttag ctcctgaaaa tctcgataac tcattaaagc
tagcggccgc tagcttatcg 2160
2161 atgataagct gtcaaacatg agaatttagc ccgcctaatg
agcgggcttt ttgttgtgat 2220
2221 ccggatcttc tcgaggtcga cggtatcgat aagcttagat
ctgtggtctc atacagaact 2280
2281 tataagattc ccaaatccaa agacatttca cgtttatggt
gatttcccag aacacatagc 2340
2341 gacatgcaaa tattgcaggg cgccactccc ctgtccctca
cagccatctt cctgccaggg 2400
2401 cgcacgcgcg ctgggtgttc ccgcctagtg acactgggcc
cgcgattcct tggagcgggt 2460
2461 tgatgacgtc agcgttcgaa ttctaccggg taggggaggc
gcttttccca aggcagtctg 2520
2521 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
cacaagtggc ctctggcctc 2580
2581 gcacacattc cacatccacc ggtaggcgcc aaccggctcc
gttctttggt ggccccttcg 2640
2641 cgccaccttc tactcctccc ctagtcagga agttcccccc
cgccccgcag ctcgcgtcgt 2700
2701 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
cgtgcagatg gacagcaccg 2760
2761 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
atagcagctt tgctccttcg 2820
2821 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
cgggctcagg ggcgggctca 2880
2881 ggggcggggc gggcgcccga aggtcctccg gaggcccggc
attctgcacg cttcaaaagc 2940
2941 gcacgtctgc cgcgctgttc tcctcttcct catctccggg
cctttcgacc tgcagcccaa 3000
3001 gctagcttac catgaccgag tacaagccca cggtgcgcct
cgccacccgc gacgacgtcc 3060
3061 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
ccccgccacg cgccacaccg 3120
3121 tcgatccgga ccgccacatc gagcgggtca ccgagctgca
agaactcttc ctcacgcgcg 3180
3181 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
cgccgcggtg gcggtctgga 3240
3241 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
gatcggcccg cgcatggccg 3300
3301 agttgagcgg ttcccggctg gccgcgcagc aacagatgga
aggcctcctg gcgccgcacc 3360
3361 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
ctcgcccgac caccagggca 3420
3421 agggtctggg cagcgccgtc gtgctccccg gagtggaggc
ggccgagcgc gccggggtgc 3480
3481 ccgccttcct ggagacctcc gcgccccgca acctcccctt
ctacgagcgg ctcggcttca 3540
3541 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
ctggtgcatg acccgcaagc 3600
3601 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
cgaaaggagc gcacgacccc 3660
3661 atgcatcgat aaaataaaag attttattta gtctccagaa
aaagggggga atgaaagacc 3720
3721 ccacctgtag gtttggcaag ctagagaacc atcagatgtt
tccagggtgc cccaaggacc 3780
3781 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
cgcttctcgc ttctgttcgc 3840
3841 gcgcttctgc tccccgagct caataaaaga gcccacaacc
cctcactcgg cgcgccagtc 3900
3901 ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat
aaaccctctt gcagttgcat 3960
3961 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
gagtgattga ctacccgtca 4020
4021 gcgggggtct ttcatgggta acagtttctt gaagttggag
aacaacattc tgagggtagg 4080
4081 agtcgaatat taagtaatcc tgactcaatt agccactgtt
ttgaatccac atactccaat 4140
4141 actcctgaaa tagttcatta tggacagcgc agaagagctg
gggagaatta attcgtaatc 4200
4201 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
acaattccac acaacatacg 4260
4261 agccggaagc ataaagtgta aagcctgggg tgcctaatga
gtgagctaac tcacattaat 4320
4321 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
tcgtgccagc tgcattaatg 4380
4381 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
cgctcttccg cttcctcgct 4440
4441 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
gtatcagctc actcaaaggc 4500
4501 ggtaatacgg ttatccacag aatcagggga taacgcagga
aagaacatgt gagcaaaagg 4560
4561 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
gcgtttttcc ataggctccg 4620
4621 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
aggtggcgaa acccgacagg 4680
4681 actataaaga taccaggcgt ttccccctgg aagctccctc
gtgcgctctc ctgttccgac 4740
4741 cctgccgctt accggatacc tgtccgcctt tctcccttcg
ggaagcgtgg cgctttctca 4800
4801 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
cgctccaagc tgggctgtgt 4860
4861 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
ggtaactatc gtcttgagtc 4920
4921 caacccggta agacacgact tatcgccact ggcagcagcc
actggtaaca ggattagcag 4980
4981 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
tggcctaact acggctacac 5040
5041 tagaaggaca gtatttggta tctgcgctct gctgaagcca
gttaccttcg gaaaaagagt 5100
5101 tggtagctct tgatccggca aacaaaccac cgctggtagc
ggtggttttt ttgtttgcaa 5160
5161 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
cctttgatct tttctacggg 5220
5221 gtctgacgct cagtggaacg aaaactcacg ttaagggatt
ttggtcatga gattatcaaa 5280
5281 aaggatcttc acctagatcc ttttaaatta aaaatgaagt
tttaaatcaa tctaaagtat 5340
5341 atatgagtaa acttggtctg acagttacca atgcttaatc
agtgaggcac ctatctcagc 5400
5401 gatctgtcta tttcgttcat ccatagttgc ctgactcccc
gtcgtgtaga taactacgat 5460
5461 acgggagggc ttaccatctg gccccagtgc tgcaatgata
ccgcgagacc cacgctcacc 5520
5521 ggctccagat ttatcagcaa taaaccagcc agccggaagg
gccgagcgca gaagtggtcc 5580
5581 tgcaacttta tccgcctcca tccagtctat taattgttgc
cgggaagcta gagtaagtag 5640
5641 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
acaggcatcg tggtgtcacg 5700
5701 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
cgatcaaggc gagttacatg 5760
5761 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
cctccgatcg ttgtcagaag 5820
5821 taagttggcc gcagtgttat cactcatggt tatggcagca
ctgcataatt ctcttactgt 5880
5881 catgccatcc gtaagatgct tttctgtgac tggtgagtac
tcaaccaagt cattctgaga 5940
5941 atagtgtatg cggcgaccga gttgctcttg cccggcgtca
atacgggata ataccgcgcc 6000
6001 acatagcaga actttaaaag tgctcatcat tggaaaacgt
tcttcggggc gaaaactctc 6060
6061 aaggatctta ccgctgttga gatccagttc gatgtaaccc
actcgtgcac ccaactgatc 6120
6121 ttcagcatct tttactttca ccagcgtttc tgggtgagca
aaaacaggaa ggcaaaatgc 6180
6181 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
ctcatactct tcctttttca 6240
6241 atattattga agcatttatc agggttattg tctcatgagc
ggatacatat ttgaatgtat 6300
6301 ttagaaaaat aaacaaatag gggttccgcg cacatttccc
cgaaaagtgc cacctgacgt 6360
6361 ctaagaaacc attattatca tgacattaac ctataaaaat
aggcgtatca cgaggccctt 6420
6421 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
cacatgcagc tcccggagac 6480
6481 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
gcccgtcagg gcgcgtcagc 6540
6541 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
tcagagcaga ttgtactgag 6600
6601 agtgcaccat atgcggtgtg aaataccgca cagatgcgta
aggagaaaat accgcatcag 6660
6661 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
cgatcggtgc gggcctcttc 6720
6721 gctattacgc cagctggcga aagggggatg tgctgcaagg
cgattaagtt gggtaacgcc 6780
6781 agggttttcc cagtcacgac gttgtaaaac gacggcgcaa
ggaatggtgc atgcaaggag 6840
6841 atggcgccca acagtccccc ggccacgggg cctgccacca
tacccacgcc gaaacaagcg 6900
6901 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
tgatgtcggc gatataggcg 6960
6961 ccagcaaccg cacctgtggc gccggtgatg ccggccacga
tgcgtccggc gtagaggcga 7020
7021 ttagtccaat ttgttaaaga caggatatca gtggtccagg
ctctagtttt gactcaacaa 7080
7081 tatcaccagc tgaagcctat agagtacgag ccatagataa
aataaaagat tttatttagt 7140
7141 ctccagaaaa aggggggaa 7159