Sequence Verified C. elegans RNAi Clones
Source BioScience LifeSciences will end sequence your
clones before they are sent to you - you only pay for the clones
that BLAST match with the id you requested. This risk free option
saves you time when you get your clone and removes the chance of
receiving one of the wrong clones that are inherent in all copies
of large libraries.
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- Place your order conventionally but follow the Sequence
Verified clones link
- Source BioScience LifeSciences will extract DNA, end sequence
your requested clone(s) and BLAST the output
- Successfully verified clones (>95% identity after quality
clipping) will be shipped to you and we will include a BLAST report
in the packet
- Customers will be notified in their delivery about the clones
that fail verification, no charge will be made for failed clones.
In the unlikely event that all clones in the order fail
verification the order will be completely cancelled at no charge
and customers will be contacted by email.
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C. elegans RNAi library
This Caenorhabditis elegans RNAi feeding library
provided by Source BioScience LifeSciences was constructed by Julie
Ahringer's group at the The Wellcome CRC Institute, University of
Cambridge, Cambridge, England.
C. elegans genomic fragments
were PCR amplified using Research Genetics GenePairs, cloned into the
EcoRV site of vector L4440 from Timmons and Fire ( Nature, 395, 854) and transformed into
bacterial strain HT115 ( Gene, 263, 103-112) as described ( Nature 408, 325-330). The whole genome library
consists of 16,757 bacterial strains, which cover 87% of C.
elegans genes.
Bacterial strains carry the GenePairs
name, which usually, but does not always correspond to a predicted
C. elegans gene name. A current mapping of GenePair to
gene can be found in WormBase (http://www.wormbase.org/). GenePairs primer
sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.
There is also a "C.elegans
Finder" tool available which allows you to find the primer
sequences for any given GenePair Name in addition to the location
of that bacterial strain in Source BioScience LifeSciences 384-well
plates. The complete C.elegans RNAi database can be
downloaded here . Note that the mapping of
some GenePairs has changed since WS56 when this database was
built.
The libraries is available by individual chromosome sets (I, II,
III, IV, V and X) from Source BioScience LifeSciences as frozen
glycerol stocks of bacterial strains arrayed in 384 well plates or
as individual bacterial strains (clones).
We are also supplying re-arrayed
sub-sets of the libraries in the following areas:
- Chromatin (257 clones)
- Phosphatase (166 clones)
- Transcription factors (387 clones).