Please note that the clone(s) being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.
THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER ). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.
What to do with your cloned when you receive them
Plates- contain LB broth with 8% glycerol and kanamycin, are sent on Dry Ice. These should be stored at -70°C
Individual clones pools have been streaked onto LB agar containing kanamycin (100µg/ml). Please store them at 4°C (not in a freezer). As soon as possible, transfer the culture to LB broth containing kanamycin + 8% glycerol and incubate overnight at 37°C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37°C for subsequent freezing at -70°C. These frozen stocks can then be used as templates for PCR amplification of ORFs, either directly in colony PCRs or as plasmid DNA.
These clones are for research purposes only.
Click here http://www.invitrogen.com/content/sfs/vectors/pdonr201_pdonr207_map.pdf to view vector map.
WormBase - http://www.wormbase.org/
Worfdb - http://worfdb.dfci.harvard.edu/
Acknowledgements and subsequent publication
We are grateful to Dr. Marc Vidal (Dana-Farber Cancer Institute, Harvard Medical School, Boston) for the gift of this library and associated database and Dr Troy Moore (Open Biosystems) for preparing a copy of the library.
Please acknowledge the Dana-Farber Cancer Institute and Source BioScience LifeSciences in any resulting publications, clones should be described in the paper in their ID form eg T22D1.3
Please also cite the following paper:
C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression: Reboul J, Vaglio P, Rual JF, Lamesch P, Martinez M, Armstrong CM, Li S, Jacotot L, Bertin N, Janky R, Moore T, Hudson JR Jr, Hartley JL, Brasch MA, Vandenhaute J, Boulton S, Endress GA, Jenna S, Chevet E, Papasotiropoulos V, Tolias PP, Ptacek J, Snyder M, Huang R, Chance MR, Lee H, Doucette-Stamm L, Hill DE, Vidal M.Nat Genet. 2003 May;34(1):35-41.