Following on from the success of the C. elegans ORFeome library, Marc Vidal and his lab, have given us access to distribute his C. elegans Promoterome library.
The development of systems biology requires the characterisation of expression profiles. Interpretation of such results should allow for the assessment of gene expression in cells and tissues, under different developmental stages and environmental conditions. The library includes clones containing ~6,000 predicted promoters, cloned into the highly flexible MultiSite Gateway™ system (Invitrogen). These promoters can be transferred easily into various Gateway ™ Destination vectors to drive expression of markers such as GFP alone (promoter::GFP constructs), or in fusion with protein-encoding open reading frames available in ORFeome resources (promoter::ORF::GFP).
Figure 1. Downstream applications of the promoterome clones.
By utilising the cloned C. elegans promterome library, a large number of in vivo assays can be performed in the nematode.
- Tissue specific RNAi.
- Sub-cellular localisation, Co-immuno-precipitation and Phenotype rescue.
- Expression mapping.
- mRNA tagging. Blue: attB site; Red: attL site; Yellow: attR site.
Source BioScience offers, for research purposes only, individual clones from a single well, a subset of clones or the complete set of 71 plates (96-well format). There's also a C. elegans Promoter finder tool available that allows you to search for your promoter of interest, using a clone ID.
We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.
Genome Res. 2004 Oct;14(10B):2169-75.
"A first version of the Caenorhabditis elegans Promoterome."
Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.
Center for Cancer Systems Biology and Department of Cancer Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.