C. elegans RNAi library

The Caenorhabditis elegans RNAi feeding library was constructed by Julie Ahringer's group at The Wellcome CRC Institute, University of Cambridge, Cambridge, UK and is distributed by Source BioScience.  It is designed for genome wide study of gene function in C. elegans through loss of function studies.  It can be used both for rapid large scale RNAi experiments and for the study of individual genes.

When produced initially the library targeted 72% of C.elegans genes (according to current gene annotation).  Julie Ahringer and her team in conjuction with Source BioScience have since generated a collection of new supplemental bacterial strains to enhance this library, and the total library now targets around 87% of currently annotated C.elegans genes. These supplemental strains are now available to order from Source BioScience.

The library is available as individual chromosome sets (I, II, III, IV, V and X) which include the new supplemental strains.   

The complete supplemental RNAi set of plates and individual chromosome sets (I, II, III, IV, V and X) are also available to order for those who have already purchased the library in the past.

Set details

Library sets

Number of plates

Approximate clone number

C.elegans Chr.I RNAi

10

2,875

C.elegans Chr.II RNAi

11

3,594

C.elegans Chr.III RNAi

8

2,630

C.elegans Chr.IV RNAi

10

3,221

C.elegans Chr.V RNAi

16

4,716

C.elegans Chr.X RNAi

8

2,726

C.elegans Chr RNAi - complete set 63 19,762

The complete C.elegans RNAi database including the new supplementary bacterial strains can be downloaded here.   

NEW supplementary sets

Number of plates

Approximate clone number

C.elegans supplementary Chr. RNAi - Chr.I

2

430

C.elegans supplementary Chr. RNAi - Chr.II

2

620

C.elegans supplementary Chr. RNAi - Chr.III

2

498

C.elegans supplementary Chr. RNAi - Chr.IV

2

528

C.elegans supplementary Chr. RNAi - Chr.V

3

1,060

C.elegans supplementary Chr. RNAi - Chr.X

1

371

C.elegans supplementary Chr. RNAi complete set

12

3,507

The library is provided as frozen glycerol stocks of bacterial strains arrayed in 384 well plates.

Individual bacterial strains (clones) are also available and can be found and ordered on GenomeCUBE.  Individual strains are available in sequence verified and purified plasmid formats.  

Library details

C. elegans genomic fragments were PCR amplified, cloned into the EcoRV site of vector L4440 from Timmons and Fire ( Nature, 395, 854) and transformed into bacterial strain HT115 ( Gene, 263, 103-112) as described ( Nature 408, 325-330).  

Further details and feeding protocol

For library and clone handling guidelines click here

To find the location of the bacterial strain in Source BioScience 384-well plates please use the database files below.

The complete C.elegans RNAi database including the new supplementary bacterial strains can be downloaded here

Note that the mapping of some primer pairs has changed since these databases were built.

A current mapping of primer pairs to genes can be found in C.elegans bioinformatics database WormBase by searching using gene name or primer pair name (http://www.wormbase.org/) and the primer pair sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.

We also supply re-arrayed sub-sets of the libraries in the following areas:

  1. Chromatin  (257 clones)
  2. Phosphatase (166 clones)
  3. Transcription factors (387 clones).

How to order

Library, plate set and individual strain orders must be placed via our website.  An MTA must be agreed to during the on-line ordering process and you can read this MTAs at the links below

Library of Bacterial Strains of C. elegans genes (academic non-USA)

Library of Bacterial Strains of C. elegans genes (academic USA)

Library of Bacterial Strains of C. elegans genes (commercial non UK)

Library of Bacterial Strains of C. elegans genes (commercial UK only)

To order the library and individual chromosome sets use GenomeCUBE. Please note that individual bacterial strains can be found on our GenomeCUBE search using their primer pair name.

If you have any questions please contact our technical team who will be happy to help you. 

 

Clone and library handling details

Ahringer Lab Protocol

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