C.elegans RNAi library plates

The bacterial strains in the 384-well library plates have been grown in LB broth containing 8% glycerol and 25µg/ml carbenicillin. The plates have been shipped to you on dry ice and should be frozen at -70°C on receipt.

Individual bacterial strains have been streaked onto LB agar containing 25µg/ml carbenicillin. The agar slopes should be stored at 4°C on receipt.

If you encounter problems growing clones, please streak for single colonies onto LB agar containing 50µg/ml Ampicillin and 15µg/ml Tetracycline.

Please see the accompanying Ahringer Lab protocol for use of this library.

The primer sequences for any given gene name  in addition to the location of that bacterial strain in Source BioScience LifeSciences 384-well plates can be found in the complete C.elegans RNAi database which can be downloaded here.   

Every effort has been made to ensure that the plates that you receive are as close as possible to the published database. However in some plates, there may be empty wells due to a clone not growing - please inform us if this happens and we will try to replace this clone if possible. In the last plate of each set, due to plates having been condensed from 96 well into 384 well plates- there may be only eg only 1 x 96 well plate resulting in 75% of the plate being empty. If any growth is observed in a well thatshould be empty, please ignore this, it may be due to very low level cross contamination.

For the re-arrayed subsets please consult the following datasheets

  1. Chromatin
  2. Phosphatase
  3. Transcription factors

C. elegans RNAi Bacterial Strains - an explanation of your despatch note

On your despatch note a bacterial strain is called a "CLONE", below is an example of a despatch note printout indicating the different IDs used:

 

c elegans rnai library plates and stains 1

Subsequent publication

 Please acknowledge the originator of the library, Dr Julie Ahringer, and Source BioScience for providing the library in any publication arising out of using this resource.

Devgen Notice

a)
The RNAi feeding library may be provided to not-for-profit and commercial entities, but for research purposes only.
For any intended commercial use of the RNAi feeding technology, your attention is drawn to the following patent applications and patents in the name of Devgen N.V., Belgium: EP-A-1 093 526, EP-A- 1 197 567, GB 2349885-B, GB 2370275-A and GB-2362885-B, and further patent applications and patents that may correspond thereto.
Source BioScience LifeSciences does not have the right to grant any license (either explicit or implied) under any patent applications and/or patents held by Devgen N.V.. Should you wish to obtain such a license, you are advised to contact Devgen N.V., tel. Belgium-9-3 24 24 24; fax Belgium-9-3 24 24 25 or e-mail: info@devgen.com .

 

b)
The RNAi feeding library is provided for research purposes only, and may not be used for any commercial purposes, including but not limited to: (1) screening by any entity other than a not-for-profit organization of the RNAi feeding library or any substantial part thereof of on average more than 30 genes per screening day; and/or (2) offering and/or providing to third parties, in return for any financial and/or other consideration, of any services relating to and/or involving the RNAi feeding library.
For any intended commercial use of the RNAi feeding technology, your attention is drawn to the following patent applications and patents in the name of Devgen N.V., Belgium: EP-A-1 093 526, EP-A- 1 197 567, GB 2349885-B, GB 2370275-A and GB-2362885-B, and further patent applications and patents that may correspond thereto.
Source BioScience LifeSciences does not have the right to grant any license (either explicit or implied) under any patent applications and/or patents held by Devgen N.V.. Should you wish to obtain such a license, you are advised to contact Devgen N.V., tel. Belgium-9-3 24 24 24; fax Belgium-9-3 24 24 25 or e-mail: info@devgen.com .