The use of cells bearing a T7 RNA polymerase gene for in vivo production of transcripts is based on technologies developed at Brookhaven National Laboratory under contract with the U. S. Department of Energy and is the subject of U.S. patents assigned to Brookhaven Science Associates, LLC. (BSA). BSA will grant a non-exclusive license for use of this technology, including the enclosed materials, based upon the following assurances:
1. These materials are to be used for noncommercial research purposes only. A separate license is required for any commercial use by commercial entities doing business in whole or in part in the United States, including the use of these materials for research purposes or production purposes by any commercial entity. Information about commercial licenses may be obtained from the Office of Intellectual Property & Industrial Partnerships, Brookhaven National Laboratory, Bldg. 475D, P. O. Box 5000, Upton, New York 11973-5000, telephone (631)-344-7134; firstname.lastname@example.org.
2. No materials that contain the cloned copy of T7 gene 1, the gene for T7 RNA polymerase, may be distributed further to third parties outside of your laboratory, unless the recipient receives a copy of this license and agrees to be bound by its terms. This limitation applies to strains BL21(DE3), BL21(DE3)pLysS, and BL21(DE3)pLysE, HT115(DE3) and any derivatives you may make of them.