This Caenorhabditis elegans RNAi feeding library provided by Source BioScience was constructed by Julie Ahringer's group at the The Wellcome CRC Institute, University of Cambridge, Cambridge, England.
C. elegans genomic fragments were PCR amplified, cloned into the EcoRV site of vector L4440 from Timmons and Fire ( Nature, 395, 854) and transformed into bacterial strain HT115 ( Gene, 263, 103-112) as described ( Nature 408, 325-330). The whole genome library consists of 16,757 bacterial strains, which cover 87% of C. elegans genes.
Bacterial strains carry the GenePairs name, which usually, but does not always correspond to a predicted C. elegans gene name. A current mapping of GenePair to gene can be found in WormBase (http://www.wormbase.org/). GenePairs primer sequences are available at http://cmgm.stanford.edu/~kimlab/primers.12-22-99.html.
You to find the primer sequences for any given gene name in addition to the location of that bacterial strain in our 384-well plates in the complete C.elegans RNAi database which can be downloaded here . Note that the mapping of some GenePairs has changed since WS56 when this database was built.
The libraries is available by individual chromosome sets (I, II, III, IV, V and X) from Source BioScience LifeSciences as frozen glycerol stocks of bacterial strains arrayed in 384 well plates or as individual bacterial strains (clones).
We are also supplying re-arrayed sub-sets of the libraries in the following areas: