Chicken BAC clones (in E. coli)

Chicken BAC clones (in E. coli)

Clones are streaked onto LB agar containing chloramphenicol (12.5µg/ml).
To use: restreak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight.

 

Single colonies are then ready for DNA isolation, if required, using the accompanying protocol which although described for PAC clones also works for BAC clones.

 

Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 25% for long term storage at -70°C.

 

pBeloBAC1 Vector

 

The vector used, pBeloBAC1, is a modification of pBeloBAC11, containing a unique EcoR1 site: the EcoR1 site within the vector having been removed leaving only one EcoR1 site within the lacZ gene (Frijters et al Theor. Appl. Genet. [1997] 94:390-399).

 

For further information on pBeloBAC11 see: http://informa.bio.caltech.edu/protocols/BAC_lib_construction.html

 

DNA isolation

 

Included is a protocol which although described for PAC clones also works for BAC clones (with the modification of changing kanamycin for chloramphenicol for the clone growth).

 

Subsequent publication:



Please acknowledge the originators of the library, Richard Crooijmans et al, and Source BioScience for providing the clone in any publication arising out of using this resource. An example of how a clone (E.g. 101-m19) should be described in a publication is: 101m19 from the Chicken BAC library.