The filters are labelled at the top left hand corner (Chicken 1, Chicken 2 and Chicken 3). This label serves as an orientation mark. The enclosed sheets [ i, and tables (ii), (iii) and (iv)] provide detailed information about the layout of clones on each filter.
There are three large filters supplied.
Sheet (i) shows a typical 22 x 22 cm membrane with 36,864 spotted clones. Each filter is divided into 6 panels (indicated by 1, 2 ......6 ). The panels are numbered from left to right and eight 384-well plates are gridded, in duplicate, in each panel. Within each panel, numbers 1-24 correspond to the 24 columns and letters A-P are the 16 rows of the eight 384-well plates in the panel. Each dot represents a clone from a single microtitre plate well. A group of 16 dots corresponds to a 4x4 array of duplicate clones from the 8 microtitre plates (8 x 2). Thus each panel consists of 6144 clones (384 x 8 x 2). Table (ii) shows the order of the 8 plates (in duplicate) within each panel on the filter. Panel 1 contains plates 1-8, panel 2 contains 9-16 and so on. This plate order has been maintained on all the membranes, e.g.
The interpretation of positive signal(s) can be done by following the procedure given below:
Use either table (ii) or table (iii) to identify which plate number is giving the positive signal.